Abstract
Most T cells bear an antigen receptor that is a protein of a disulphide-linked heterodimer composed of an a chain and a β chain associated with the non-polymorphic CD3 (T3) complex1–7. A small subpopulation of thymic and peripheral T cells8–17, as well as Thy-1 +dendritic epidermal cells (dEC) (refs 18–21), express an alternative CD3-associated dimeric receptor composed of the product of the T-cell antigen receptor (TCR) γ gene22 and a fourth chain, designated δ. Recently a new murine TCR constant-region gene, designated Cx, has been cloned and proposed as a candidate for the Cδ gene23. We have previously demonstrated that murine Thy-1+ dEC cell lines express a CD3-associated disulphide-linked heterodimer composed of a relative molecular mass Mr 41,000 (41K) γ chain and a 50K δ chain21. We have further analysed the receptor of one of these cloned dEC lines, 7–17.1, by endoglycosidase treatment of the isolated γ and δ chains. The γ chain was found to contain two N-linked oligosaccharide residues, consistent with the expression of a chain encoded by the Vγ3 and Cγ1 gene segments. The δ chain contains at least three N-linked oligosaccharides and has a core size of 38K. Northern blot analysis indicated the presence of abundant Cx messenger RNA in 7–17.1 cells. Immunoprecipitation with two antisera to peptides comprising distinct regions of the Cx sequence indicates that the δ chain is encoded by the Cx gene.
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References
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Bonyhadi, M., Weiss, A., Tucker, P. et al. Delta is the Cx-gene product in the γ/δ antigen receptor of dendritic epidermal cells. Nature 330, 574–576 (1987). https://doi.org/10.1038/330574a0
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DOI: https://doi.org/10.1038/330574a0
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