Abstract
Although most mature peripheral T lymphocytes express a major histocompatibility complex restricted, CD3-associated, antigen receptor (TCR) which has been well characterized1–16, some T cells carry a different CD3-associated heterodimer on their surface17–22. One of the two disulphide-linked chains of this putative second receptor, which in mice has relative molecular mass (Mr) 35,000 (35K), has been identified as a product of the group of γ genes18. The other chain, termed δ (Mr 45K in mice), is not as well characterized. Although γ/δ-bearing cells are a minor subset among peripheral T lymphocytes, they are the only CD3+ cells in the thymus early in ontogeny19. Taking advantage of these kinetics, we have generated γ/δ-bearing hybridomas, using a new TCR α chain-negative variant of the AKR thymoma BW5147 as tumour parent, fetal thymocytes as normal cell partners, and an anti-CD3 monoclonal antibody (mAb) as screening reagent23. Gamma and δ chains from one of these hybrids have been purified and partially sequenced. The sequences obtained indicate that δ is indeed identical to the polypeptide encoded by the recently described gene X, as suggested by Chien et al.24.
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Born, W., Miles, C., White, J. et al. Peptide sequences of T-cell receptor δ and γ chains are identical to predicted X and γ proteins. Nature 330, 572–574 (1987). https://doi.org/10.1038/330572a0
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DOI: https://doi.org/10.1038/330572a0
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