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Myostatin antisense RNA-mediated muscle growth in normal and cancer cachexia mice

Gene Therapy volume 15, pages 155–160 (2008)Cite this article

Abstract

Myostatin is a negative regulator of myogenesis, and inactivation of myostatin leads to muscle growth. Here we have used modified RNA oligonucleotides targeting the myostatin mRNA and examined the therapeutic potential in normal and cancer cachexia mouse models. We found that the RNA oligonucleotides could suppress the myostatin expression in vivo, leading to the increase in muscle growth both in normal and cachectic mice. We also established that the effect of myostatin inhibition caused by the RNA oligonucleotides may be through the MyoD pathway, as evidenced by a significant upregulation of MyoD expression. Taken together, these results demonstrate the feasibility using antisense strategy for the treatment of muscle wasting conditions.

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Author notes

  1. C-M Liu and Z Yang: These authors contributed equally to this work.

Authors and Affiliations

  1. Molecular Virology Research Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

    C-M Liu, Z Yang, C-W Liu, R Wang & P Tien

  2. Oligos Etc Inc., Wilsonville, OR, USA

    R Dale & L-Q Sun

Authors
  1. C-M Liu
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  2. Z Yang
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  3. C-W Liu
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  4. R Wang
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  5. P Tien
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  6. R Dale
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  7. L-Q Sun
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Correspondence to P Tien or L-Q Sun.

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Liu, CM., Yang, Z., Liu, CW. et al. Myostatin antisense RNA-mediated muscle growth in normal and cancer cachexia mice. Gene Ther 15, 155–160 (2008). https://doi.org/10.1038/sj.gt.3303016

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  • DOI: https://doi.org/10.1038/sj.gt.3303016

Keywords

  • myostatin
  • antisense
  • muscle wasting
  • cancer cachexia

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