Abstract
Microtubules are involved in several forms of intracellular motility, including mitosis and organelle movement. Fast axonal transport is a highly ordered form of organelle motility that operates in both the anterograde (outwards from the cell body) and retrograde (from the periphery towards the cell body) direction1. Similar microtubule-associated movement is observed in non-neuronal cells2, and might be involved in secretion, endocytosis and the positioning of organelles within the cell3,4. Kinesin5 is a mechanochemical protein that produces force along microtubules in an anterograde direction6. We recently found that the brain microtubule-associated protein MAP 1C (ref. 7) is a microtubule-activated ATPase8 and, like kinesin, can translocate microtubules in an in vitro assay for microtubule-associated motility8. MAP 1C seemed to be related to the ciliary and flagellar ATPase, dynein, which is thought to produce force in a direction opposite to that observed for kinesin9. Here we report that MAP 1C, in fact, acts in a direction opposite to kinesin, and has the properties of a retrograde translocator.
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References
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Paschal, B., Vallee, R. Retrograde transport by the microtubule-associated protein MAP 1C. Nature 330, 181–183 (1987). https://doi.org/10.1038/330181a0
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DOI: https://doi.org/10.1038/330181a0
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