Abstract
We evaluated the efficiency of adenovirus-mediated gene transfer into normal and malignant human hematopoietic cells. An E-1 and E-3 deleted, replication-defective recombinant Ad.RSVβgal vector was used and the transduction efficiency was studied at a multiplicity of infection of 13 p.f.u. per cell. Approximately 40–50% of normal monocytes were transduced, whereas purified normal resting T cells and B cells were resistant to infection. We showed that 50–80% of primary chronic myeloid leukemia cells (CML, n = 12) were efficiently transduced. In contrast to CML, successful transduction of resting primary chronic B lymphocytic leukemia cells required appropriate preactivation of targeted cells. A novel protocol for the efficient transduction of adenovirus into B-CLL cells was presented. We showed that anti-CD40 mAb or CD40 ligand acts in synergy with rhIL-4 to enable the transduction of approximately 50–75% of B-CLL cells (B-CLL, n = 6). Expression of β-galactosidase in transduced CML cells and B-CLL cells was detected for at least 15 days after transduction. The present studies underline the utility of adenovirus vectors for the construction of cytokine gene-modified tumor vaccines for the treatment of hematopoietic malignancies such as CML and B-CLL.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 12 print issues and online access
$259.00 per year
only $21.58 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huang, M., Olsson, M., Kallin, A. et al. Efficient adenovirus-mediated gene transduction of normal and leukemic hematopoietic cells. Gene Ther 4, 1093–1099 (1997). https://doi.org/10.1038/sj.gt.3300499
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/sj.gt.3300499
Keywords
This article is cited by
-
Efficient gene transfer in CLL by mRNA electroporation
Leukemia (2008)
-
Isolation and transduction of monocytes: promising vehicles for therapeutic arteriogenesis
Langenbeck's Archives of Surgery (2006)
-
Purging of leukemia-contaminated bone marrow grafts using suicide adenoviral vectors: an in vivo murine experimental model
Gene Therapy (2003)
-
Recombinant adenovirus-mediated cytotoxic gene therapy of lymphoproliferative disorders: is CAR important for the vector to ride?
Gene Therapy (2003)
-
Gene-based cancer vaccines: an ex vivo approach
Leukemia (2001)