Abstract
Attempts to use X-ray crystallography to extract three-dimensional information on transient phenomena in crystals have been hampered primarily by long data collection times. Here we report on the first difference Fourier map obtained from Laue diffraction1–8 photographs of a protein crystal, glycogen phosphorylase b. Data collection time was 3 s using the high-intensity white X-radiation generated on the wiggler magnet9 of the Daresbury Synchrotron Radiation Source (SRS), but data acquisition in the millisecond-submillisecond range is possible. The method presented here uses a simple difference technique and was designed to analyse structural changes relative to a known starting structure. The combination of this approach with cine techniques allows the recording of three-dimensional motion pictures at atomic resolution and opens up new areas in structural biology and chemistry.
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Hajdu, J., Machin, P., Campbell, J. et al. Millisecond X-ray diffraction and the first electron density map from Laue photographs of a protein crystal. Nature 329, 178–181 (1987). https://doi.org/10.1038/329178a0
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DOI: https://doi.org/10.1038/329178a0
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