Letter | Published:

DNA fingerprinting in birds

Naturevolume 327pages149152 (1987) | Download Citation

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Abstract

Several regions of the human genome are highly variable in populations because the number of repeats in these regions of a short 'minisatellite' sequence varies at high frequency. Different minisatellites have a core sequence1,2 in common, however, and probes made up of tandem repeats of this core sequence detect many highly variable DNA fragments in several species including humans1,3, cats4, dogs4 and mice5. The hypervariable sequences detected in this way are dispersed in the genome and their variability means that they can be used as a DNA 'fingerprint', providing a novel method for the identification of individuals2,6, confirmation of biological relationships7,8 and human genetic analysis9,10. We show here that human minisatellite-derived probes also detect highly variable regions in bird DNAs. Segregation analysis in a house sparrow family confirms that these regions comprise many mostly heterozygous dispersed loci and we conclude that house sparrow DNA fingerprints are analagous to those of humans. Fingerprint analysis identified one nestling, with fingerprint bands not present in the parent pair's fingerprints, which we conclude resulted from an extrapair copulation. Extrabond copulations have been described in many wild bird species11–13, but their success and hence adaptive significance have rarely been quantifiable14–20. DNA fingerprinting will be of great significance to studies of the sociobiology, demography and ecology of wild birds.

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References

  1. 1

    1. Jeffreys, A. J., Wilson, V. & Thein, S. L. Nature 314, 67–73 (1985). 2. Jeffreys, A. J., Wilson, V. & Thein, S. L. Nature 316, 76–79 (1985). 3. Wong, Z. & Jeffreys, A. J. Nucleic Acids Res. 14, 4605–4616 (1986). 4. Jeffreys, A. J. & Morton, D. B. Anim. Genet. 18 (in the press). 5. Jeffreys, A. J., Wilson, V., Kelly, R. Taylor, B. A. & Bulfield, G. Nucleic Acids Res. (in the press). 6. Gill, P., Jeffreys, A. J. & Werrett, D. J. Nature 318, 577–579 (1985). 7. Jeffreys, A. J., Brookfield, J. F. Y. & Semeonoff, R. Nature 317, 818–819 (1985). 8. Hill, A. V. S. & Jeffreys, A. J. Lancet i, 1394–1395 (1985). 9. Jeffreys, A. J., Wilson, V., Thein, S. L., Weatherall, D. J. & Ponder, B. A. J. Am. J. hum. Genet. 39, 11–24(1986). 10. Jeffreys, A. J. Biochem. Soc. Trans. (in the press). 11. Gladstone, D. E. Am. Nat. 114, 545–557 (1979). 12. Ford, N. L. Current Ornithology 1, 330–356 (1983). 13. McKinney, F., Cheng, K. M. & Bruggers, D. J. in Sperm Competition and the Evolution of Animal Mating Systems (ed. Smith, R. L.) (Academic, New York, 1984). 14. Burns, J. T., Cheng, K. M. & McKinney, F. Auk 97, 875–879 (1980). 15. Cheng, K. M., Burns, J. T. & McKinney, F. Auk 100, 302–310 (1983). 16. Alatalo, R. V., Gustafsson, L. & Lundberg, A. Oikos 42, 41–47 (1984). 17. Burke, T. thesis, Univ. Nottingham (1984). 18. Gavin, T. A. & Bollinger, E. K. Auk 102, 550–555 (1985). 19. Westneat, D. F. Anim. Behav. (in the press). 20. Quinn, T. W., Quinn, J. S., Cooke, F. & White, B. N. Nature 326, 392–394 (1987). 21. Hill, W. G. Nature 322, 290–291 (1986).

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Affiliations

  1. Department of Zoology, University of Leicester, Leicester, LEI 7RH, UK

    • T. Burke
    •  & M. W. Bruford

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https://doi.org/10.1038/327149a0

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