Abstract
Synapsin I is a neuron-specific phosphoprotein localized to the cytoplasmic surface of synaptic vesicles1,2. This phosphoprotein is a major substrate for cyclic AMP-dependent and calcium/calmodulin-dependent protein kinases3–5. Its state of phosphorylation can be altered both in vivo and in vitro by a variety of physiological and pharmacological manipulations known to affect synaptic function6,7. Recent direct evidence suggests that it may be involved in the regulation of neurotransmitter release from the nerve terminal8. In the nerve terminal, synaptic vesicles are embedded in a cytoskeletal network, consisting in part of actin9–11. We report here the ability of the dephospho-form of synapsin I to bundle F-actin. This bundling activity is reduced when synapsin I is phosphorylated by cAMP-dependent protein kinase and virtually abolished when it is phosphorylated by calcium/calmodulin-dependent protein kinase II or by both kinases. These results, demonstrating an interaction of synapsin I with actin in vitro, support the possibility that synapsin I is involved in clustering of synaptic vesicles at the presynaptic terminal and that the phosphorylation of synapsin I may be involved in regulating the translocation of synaptic vesicles to their sites of release.
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References
DeCamilli, P., Harris, J. M. Jr, Huttner, W. B. & Greengard, P. J. Cell Biol. 96, 1355–1373 (1983).
Huttner, W. B., Schiebler, W., Greengard, P. & DeCamilli, P. J. Cell Biol. 96, 1374–1388 (1983).
Johnson, E. M., Ueda, T., Maeno, H. & Greengard, P. J. biol. Chem. 247, 5650–5662 (1972).
Huttner, W. B. & Greengard, P. Proc. natn. Acad. Sci. U.S.A. 76, 5402–5406 (1979).
Kennedy, M. B. & Greengard, P. Proc. natn. Acad. Sci. U.S.A. 78, 1293–1297 (1981).
Dolphin, A. C. & Greengard, P. Nature 289, 76–79 (1981).
Nestler, E. J. & Greengard, P. in Protein Phosphorylation in the Nervous System (Wiley, New York, 1984).
Llinas, R., McGuinness, T. L., Leonard, C. S., Sugimori, M. & Greengard, P. Proc. natn. Acad. Sci. U.S.A. 82, 3035–3039 (1985).
Toh, B. H. et al. Nature 264, 648–650 (1976).
Walker, J. H. et al. Eur. J. Cell Biol. 38, 123–133 (1985).
LeBeux, Y. & Willemot, J. Cell Tiss. Res. 160, 37–68 (1975).
Siegel, D. L. & Branton, D. J. Cell Biol. 100, 775–785 (1985).
Schiebler, W., Jahn, R., Doucet, J. P., Rothlein, J. & Greengard, P. J. biol. Chem. 261, 8383–8390 (1986).
Calissano, P., Monaco, G., Castellani, G., Mercanti, D. & Levi, A. Proc. natn. Acad. Sci. U.S.A. 75, 2210–2214 (1978).
Griffith, L. M. & Pollard, T. D. J. biol. Chem. 257, 9135–9142 (1982).
Fowler, W. E. & Aebi, U. J. Cell Biol. 93, 452–458 (1982).
DeCamilli, P. & Greengard, P. Biochem. Pharmacol. 35, 4349–4357 (1986).
Baines, A. J. & Bennett, V. Nature 315, 410–413 (1985).
Baines, A. J. & Bennett, V. Nature 319, 145–147 (1986).
Goldenring, J. R. et al. J. biol. Chem. 26, 8495–8504 (1986).
Eder, P. S., Soong, C. J. & Tao, M. Biochemistry 25, 1764–1770 (1986).
Pinder, J. C., Ohanian, V. & Gratzer, W. B. FEBS Lett. 169, 161–164 (1984).
Ungewickell, E., Bennett, P. M., Calvert, R., Ohanian, V. & Gratzer, W. B. Nature 280, 811–814 (1979).
Fowler, V. & Taylor, D. L. J. Cell Biol. 85, 361–376 (1980).
Roots, B. I. Science 221, 971–972 (1983).
Spudich, J. A. & Watt, S. J. biol. Chem. 246, 4866–4871 (1971).
MacLean-Fletcher, S. & Pollard, T. D. Biochem. biophys. Res. Commun. 96, 18–27 (1980).
Kaczmarek, L. K. et al. Proc. natn. Acad. Sci. U.S.A. 77, 7487–7491 (1980).
McGuinness, T. L., Lai, Y. & Greengard, P. J. biol. Chem. 260, 1696–1704 (1985).
Watterson, D. M., Harrelson, W. G., Keller, P. M., Sharief, F. & Vanaman, T. C. J. biol. Chem. 251, 4501–4513 (1976).
Ando, T. & Scales, D. J. biol. Chem. 260, 2321–2327 (1985).
Wilkins, J. A. & Lin, S. J. Cell Biol. 102, 1085–1092 (1986).
Kouyama, T. & Mihashi, K. Eur. J. Biochem. 114, 33–38 (1981).
Cooper, J. A., Walker, J. B. & Pollard, T. D. J. Muscle Res. Cell Mot. 4, 253–262 (1983).
Jahn, R., Schiebler, W. & Greengard, P. Proc. natn. Acad Sci. U.S.A. 81, 1684–1687 (1984).
Benfenati, F. & Guardabasso, V. in Principles and Methods in Receptor Binding (eds Cattabeni, F. & Nicosia, S.) 41–63 (Plenum, New York, 1984).
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Bähler, M., Greengard, P. Synapsin I bundles F-actin in a phosphorylation-dependent manner. Nature 326, 704–707 (1987). https://doi.org/10.1038/326704a0
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DOI: https://doi.org/10.1038/326704a0
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