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Stimulation of specific GTP binding and hydrolysis activities in lymphocyte membrane by interleukin-2

Nature volume 325, pages 166–168 (1987)Cite this article

Abstract

Interleukin-2 (IL-2) is a polypeptide growth factor which stimulates the proliferation and differentiation of T lymphocytes1,2. The receptor for IL-2 is expressed on activated T lymphocytes, cloned IL-2 dependent cells and several other cell types. Analysis of the primary structure and of immune-precipitated receptor suggests that this molecule has no intrinsic signal transduction function, unlike other growth factors. IL-2 interaction with a high affinity receptor3 has been shown, however, to activate the calcium/phos-pholipid-dependent protein kinase C (PK-C)4,5 presumably via phosphoinositide hydrolysis. Members of a family of closely related guanine nucleotide binding proteins (G proteins) regulate a diverse group of metabolic events6,7. Two of them, Gs and Gi, stimulate and inhibit adenylate cyclase activity respectively8–11, and other G proteins are involved in diverse signal transduction systems12–16. Another member, Go, has no known function and activation of phospholipase C has been attributed to the action of an unidentified G protein, GP17–19. Since it has been observed that IL-2 inhibits the catalytic activity of adenylate cyclase and that agents such as PGE2 which stimulate adenylate cyclase activity inhibit the lymphoproliferative response to IL-220, association of GTP binding proteins with IL-2 signal transduction was investigated. In this report we describe for the first time the participation of a GTP binding protein in the action of a polypeptide growth factor, interleukin-2.

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Author information

Authors and Affiliations

  1. Laboratory of Molecular Immunoregulation, National Cancer Institute, Division of Cancer Treatment, Biological Response Modifiers Program, Frederick Cancer Research Facility, Frederick, Maryland, 21701, USA

    Stuart W. Evans & William L. Farrar

  2. Program Resources Inc., National Cancer Institute, Division of Cancer Treatment, Biological Response Modifiers Program, Frederick Cancer Research Facility, Frederick, Maryland, 21701, USA

    Suzanne K. Beckner

Authors
  1. Stuart W. Evans
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  2. Suzanne K. Beckner
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  3. William L. Farrar
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Evans, S., Beckner, S. & Farrar, W. Stimulation of specific GTP binding and hydrolysis activities in lymphocyte membrane by interleukin-2. Nature 325, 166–168 (1987). https://doi.org/10.1038/325166a0

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