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High frequency of unequal recombination in pseudoautosomal region shown by proviral insertion in transgenic mouse

Nature volume 324, pages 682–685 (1986)Cite this article

Abstract

The mammalian X and Y chromosomes, in contrast to the autosomes, pair during male meiosis only near the telomeres. Alleles localized in this region can undergo reciprocal exchange during meiosis1–4. Because such sequences do not show strict sex-linked inheritance, they have been termed pseudoautosomal5. In man, several DNA sequences have been described which show pseudoautosomal transmission and which are localized in the pairing region at the ends of the short arms of both the X and Y chromosomes (refs 6–9, and D. Page, unpublished results). We now show that the transgenic mouse strain, Mov-15, contains a single Moloney murine leukaemia virus (M-MuLV) genome in its germline, and genetic evidence indicates that the provirus is integrated into the pseudoautosomal region of the sex chromosome. Proviral copies are lost or gained in 7% of male meioses in this strain, and mouse sequences flanking the provirus are tandemly repeated and highly variable. We conclude that unequal recombination events occur with high frequency in the pairing region, possibly because of the presence of repeated sequences.

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Authors and Affiliations

  1. Heinrich-Pette-Institut für Experimentelle Virologie und Immunologie an der Universität Hamburg, Martinistrasse 52, 2000, Hamburg, 20, FRG

    Klaus Harbers & Ursula Müller

  2. Whitehead Institute for Biomedical Research and Department of Biology, Massachusetts Institute of Technology, Nine Cambridge Center, Cambridge, Massachusetts, 02142, USA

    Philippe Soriano & Rudolf Jaenisch

Authors
  1. Klaus Harbers
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  2. Philippe Soriano
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  3. Ursula Müller
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  4. Rudolf Jaenisch
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Harbers, K., Soriano, P., Müller, U. et al. High frequency of unequal recombination in pseudoautosomal region shown by proviral insertion in transgenic mouse. Nature 324, 682–685 (1986). https://doi.org/10.1038/324682a0

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