Abstract
Three splicing complexes formed with a yeast pre-messenger RNA during in vitro splicing can be resolved by non-denaturing gel electrophoresis after incubation in the presence of non-specific competitor RNA. The time course of the appearance of these complexes and their composition suggest that they represent an ordered pathway of splicing complex assembly.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ruskin, B., Krainer, A. R., Maniatis, T. & Green, M. R. Cell 38, 317–331 (1984).
Padgett, R. A., Konarska, M., Grabowski, P. J., Hardy, S. F. & Sharp, P. A. Science 225, 898–903 (1984).
Konarska, M. M., Grabowski, P. J., Padgett, R. A. & Sharp, P. A. Nature 313, 552–557 (1985).
Lin, R-J., Newman, A. J., Cheng, S.-C. & Abelson, J. J. biol. Chem. 260, 14780–14792 (1985).
Newman, A. J., Lin, R.-J., Cheng, S.-C. & Abelson, J. Cell 42, 335–344 (1985).
Domdey, H. et al. Cell 39, 611–621 (1984).
Rodriguez, J. R., Pikielny, C. W. & Rosbash, M. Cell 39, 603–610 (1984).
Zeitlin, S. & Efstratiadis, A. Cell 39, 589–602 (1984).
Padgett, R. A., Grabowski, P. J., Konarska, M. M., Seiler, S. & Sharp, P. A. A. Rev. Biochem. 55, 1119–1150 (1986).
Green, M. R. A. Rev. Genetics 20, 671–708 (1986).
Rymond, B. C. & Rosbash, M. Nature 317, 735–737 (1985).
Ruskin, B., Pikielny, C. W., Rosbash, M. & Green, M. R. Proc. natn. Acad. Sci. U.S.A. 83, 2022–2026 (1986).
Pikielny, C. W. & Rosbash, M. Cell 45, 869–877 (1986).
Brody, E. & Abelson, J. Science 228, 963–967 (1985).
Grabowski, P. J., Seiler, S. R. & Sharp, P. A. Cell 42, 345–353 (1985).
Frendewey, D. & Keller, W. Cell 42, 355–367 (1985).
Bindereif, A. & Green, M. R. Molec. cell. Biol. 6, 2582–2592 (1986).
Vijayraghavan, U. et al. EMBO J. 5, 1683–1695 (1986).
Reddy, R. & Busch, H. in Progress in Nucleic Acids Research and Molecular Biology 30, (Cohn, W. E., ed.) 127–162 (Academic, New York, 1983).
Cheng, S.-C. & Abelson, J. Proc. natn. Acad. Sci. U.S.A. 83, 2387–2391 (1986).
Jacquier, A. & Rosbash, M. Proc. natn. Acad. Sci. U.S.A. 83, 5835–5839 (1986).
Pikielny, C. W., Teem, J. L. & Rosbash, M. Cell 34, 395–403 (1983).
Riedel, N., Wise, J. A., Swerdlow, H., Mak, A. & Guthrie, C. Proc. natn. Acad. Sci. U.S.A. 83, 8097–8101 (1986).
Ares, M. Jr. Cell 47, 49–59 (1986).
Mount, S. M., Pettersson, I., Hinterberger, M., Karmas, A. & Steitz, J. A. Cell 33, 509–518 (1983).
Black, D. L., Chabot, B. & Steitz, J. A. Cell 42, 737–750 (1985).
Chabot, B., Black, D. L., LeMaster, D. M. & Steitz, J. A. Science 230, 1344–1349 (1985).
Ruskin, B. & Green, M. R. Cell 43, 131–142 (1985).
Melton, D. A. et al. Nucleic Acids Res. 12, 7035–7056 (1984).
Pikielny, C. W. & Rosbach, M. Cell 41, 119–126 (1985).
England, T. E., Bruce, A. G. & Uhlenbeck, O. C. Meth. Enzym. 65, 65–74 (1980).
Bringmann, P. et al. J. biol. Chem. 258, 2745–2747 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Pikielny, C., Rymond, B. & Rosbash, M. Electrophoresis of ribonucleoproteins reveals an ordered assembly pathway of yeast splicing complexes. Nature 324, 341–345 (1986). https://doi.org/10.1038/324341a0
Published:
Issue Date:
DOI: https://doi.org/10.1038/324341a0
This article is cited by
-
Mutations in FAM50A suggest that Armfield XLID syndrome is a spliceosomopathy
Nature Communications (2020)
-
Mechanistic insights into precursor messenger RNA splicing by the spliceosome
Nature Reviews Molecular Cell Biology (2017)
-
Diverse regulation of 3′ splice site usage
Cellular and Molecular Life Sciences (2015)
-
Spliceostatin A targets SF3b and inhibits both splicing and nuclear retention of pre-mRNA
Nature Chemical Biology (2007)
-
Proteomic analysis identifies a new complex required for nuclear pre-mRNA retention and splicing
The EMBO Journal (2004)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
