Abstract
Proliferation and maturation of antigen-stimulated B cells are regulated by several soluble factors derived from macrophages and T cells1,2. These soluble factors are functionally divided into two groups: B-cell growth factor (BCGF), thought to be involved in B-cell proliferation; and B-cell differentiation factor (BCDF), responsible for maturation of activated B cells into immunoglobulin-secreting cells3–6. This classification needs to be re-examined in the light of the recent cloning of complementary DNA encoding IgG1 induction factor (interleukin-4, IL-4) from the 2.19 mouse T-cell line7. Recombinant IL-4 has BCGF and BCDF activities and affects B cells, T cells and mast cells (refs 7, 8; our unpublished data). Another well-characterized B-cell factor is T-cell replacing factor (TRF)9–12, which, when secreted by the murine T-cell hybridoma B151K12, is defined by two activities10–12: induction of IgM secretion by BCL1 leukaemic B-cell line; and induction of secondary anti-dinitrophenol (DNP) immunoglobulin G (IgG) synthesis in vitro by DNP-primed B cells. Although TRF from B151K12 was classified as BCDF, purified TRF has BCGF-II activity13. To elucidate the molecular properties of TRF we isolated cDNA encoding TRF from the 2.19 T-cell line and report here the structure and multiple activities of this lymphokine.
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References
Dutton, R. W., Folkoff, R. & Hirst, J. A. Prog. Immun. 1, 355–368 (1971).
Swain, S. L. et al. J. exp. Med. 158, 822–835 (1983).
Howard, M., Nankanishi, K. & Paul, E. W. Immun. Rev. 78, 185–210 1984).
Kishimoto, T. A. Rev. Immun. 3, 133–157 (1985).
Isakson, P. C., Puré, E., Vitetta, E. S. & Krammer, P. H. J. exp. Med. 155, 734–748 (1982).
Bergstedt-Lindqvist, S., Sideras, P., MacDonald, H. R. & Severinson, E. Immun. Rev. 78, 25–50 1984).
Noma, Y. et al. Nature 319, 640–646 (1986).
Lee, F. et al. Proc. natn. Acad. Sci. U.S.A. 83, 2061–2065 (1986).
Schimpl, A. & Wecker, E. Nature new Biol. 237, 15–17 (1972).
Takatsu, K., Tominaga, A. & Hamaoka, T. J. Immun. 124, 2414–2422 (1980).
Takatsu, K. et al. J. Immun. 125, 2646–2653 (1980).
Takatsu, K. et al. J. Immun. 134, 382–389 (1985).
Harada, N. et al J. Immun. 134, 3944–3951 (1985)
Sideras, P., Bergstedt-Lindqvist, S., MacDonald, H. R. & Severinson, E. Eur. J. Immun. 15, 586–593 (1985).
Tominaga, A., Matsumoto, M., Takahashi, T., Harada, N. & Takatsu, K. Microbiol. Immun. (in the press).
Yanisch-Perron, C., Vieira, J. & Messing, J. Gene 33, 103–119 (1985).
Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5469(1977).
Sanderson, C., O'Garra, A., Warren, J. D. & Klaus, G. B. G. Proc. natn. Acad. Sci. U.S.A. 83, 437–440 (1986).
Gronowicz, E., Coutinho, A. & Melchers, F. Eur. J. Immun. 6, 588–590 (1976).
Mage, M. G., McHugh, L. L. & Rothstein, T. L. J. immun. Meth. 15, 47–56 (1977).
Gough, M. N. et al. Nature 309, 763–767 (1984).
Gray, W. P. & Goeddel, V. D. Proc. natn. Acad. Sci. U.S.A. 80, 5842–5846 (1983).
Fung, C. M. et al. Nature 307, 233–237 (1984).
Yokota, T. et al. Proc. natn. Acad. Sci. U.S.A. 81, 1070–1074 (1984).
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Kinashi, T., Harada, N., Severinson, E. et al. Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor II. Nature 324, 70–73 (1986). https://doi.org/10.1038/324070a0
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DOI: https://doi.org/10.1038/324070a0
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