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Expression of a transfected human c-myconcogene inhibits differentiation of a mouse erythroleukaemia cell line

Nature volume 322, pages 748–750 (1986)Cite this article

Abstract

The Friend-virus-derived1 mouse erythroleukaemia (MEL) cell lines represent transformed early erythroid precursors that can be induced to differentiate into more mature erythroid cells by a variety of agents including dimethyl sulphoxide (DMSO)2There is a latent period of 12 hours after inducer is added, when 80–90% of the cells become irreversibly committed to the differentiation programme, undergoing several rounds of cell division before permanently ceasing to replicate3,4. After DMSO induction, a biphasic decline in steady-state levels of c-myc5,6 and c-myb6 messenger RNAs occurs. Following the initial decrease in c-myc mRNA expression, the subsequent increase occurs in, and is restricted to, the Gl phase of the cell cycle7. We sought to determine whether the down-regulation is a necessary step in chemically induced differentiation. Experiments reported here indicate that expression in MEL cells of a transf ected human c-myc gene inhibits the terminal differentiation process.

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Authors and Affiliations

  1. NCI-Navy Medical Oncology Branch, National Cancer Institute, Uniformed Services University of the Health Sciences, National Navy Medical Center, Bethesda, Maryland, 20814-2015, USA

    Ethan Dmitrovsky, W. Michael Kuehl, Gregory F. Hollis, Ilan R. Kirsch, Timothy P. Bender & Shoshana Segal

  2. Department of Medicine, Uniformed Services University of the Health Sciences, National Navy Medical Center, Bethesda, Maryland, 20814-2015, USA

    Shoshana Segal

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  1. Ethan Dmitrovsky
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  2. W. Michael Kuehl
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  4. Ilan R. Kirsch
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  5. Timothy P. Bender
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  6. Shoshana Segal
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Dmitrovsky, E., Kuehl, W., Hollis, G. et al. Expression of a transfected human c-myconcogene inhibits differentiation of a mouse erythroleukaemia cell line. Nature 322, 748–750 (1986). https://doi.org/10.1038/322748a0

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