Abstract
Skeletal muscle of adult, metamorphosed urodele amphibians regenerates despite the absence of satellite cells1, the myogenic reserve cells present beneath the external lamina of muscle fibres in larval urodeles and in other vertebrates2–9. Adult-stage muscles of several urodeles examined (Notophthalmus viridescens1,10, Ambystoma maculatum10, Triturus vulgaris, Triturus cristatus11, Hynobius tokyoensis12 and Ambystoma mexicanum13) have instead a unique cell type enveloped in its own external lamina. Several studies suggest that these cells are larval satellite cells that have become completely surrounded by a segment of external lamina during metamorphosis10–12. Although Popiela10 termed these cells ‘pericytes’, he suggested that they might function as a myogenic reserve population. Cherkasova, who was the first to recognize these cells as distinct from vascular pericytes, termed them post-satellite cells and also proposed that they might be myogenic11. Here, we present evidence that post-satellite cells in adult N. viridescens give rise to regenerated myotubes in vitro. The myotubes could be stained with MF20, a monoclonal antibody to purified skeletal muscle myosin14, and also with 22/18, a monoclonal antibody that reacts with an antigen present in newt-limb blastema myogenic cells15. The presence of myogenic post-satellite cells in adult newt muscle provides an alternative to the hypothesis that myoblasts in regenerating adult urodele limbs arise through dedifferentiation of mature myofibres15–17.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Hay, E. D. & Doyle, C. M. Anat. Rec. 175, 339 (1973).
Mauro, A. J. biophys. biochem. Cytol. 9, 493–495 (1961).
Carlson, B. M. in Muscle Regeneration (ed. Mauro, A.) 57–81 (Raven, New York, 1979).
Kahn, E. B. & Simpson, S. B. Devl Biol. 37, 219–223 (1974).
Konigsberg, U. R., Lipton, B. H. & Konigsberg, I. R. Devl Biol. 45, 260–275 (1975).
Bischoff, R. Anat. Rec. 180, 645–662 (1975).
Bischoff, R. in Plasticity of Muscle (ed. Pette, D.) 119–129 (Walter de Gruyter, Berlin, 1980).
Snow, M. H. Cell Tissue Res. 186, 535–540 (1978).
Campion, D. R. Int. Rev. Cytol. 87, 225–251 (1984).
Popiela, H. J. exp. Zool. 198, 57–64 (1976).
Cherkasova, L. V. Dok. Akad. Nauk. SSSR. 267, 1235–1236 (1982).
Takahama, H., Mizuhira, V., Sasaki, F. & Watanabe, K. Cell Tissue Res. 236, 431–438 (1984).
Cherkasova, L. V. Dokl. Akad. Nauk. SSSR 273, 991–993 (1983).
Bader, D., Masaki, T. & Fischman, D. A. J. Cell Biol. 95, 763–770 (1982).
Kintner, C. R. & Brockes, J. P. Nature 308, 67–69 (1984).
Lentz, T. L. Am. J. Anat. 124, 447–480 (1969).
Hay, E. D. in Concepts of Development (eds Lash, J. & Whittaker, J. R.) 404–428 (Sinauer Associates, Stamford, Connecticut, 1974).
Cameron, J. A. in Limb Development and Regeneration Pt A (eds Fallen, J. F. & Caplan, A. I.) 491–500 (Liss, New York, 1983).
Schrag, J. A. & Cameron, J. A. J. Embryol. exp. Morph. 77, 255–271 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Cameron, J., Hilgers, A. & Hinterberger, T. Evidence that reserve cells are a source of regenerated adult newt muscle in vitro. Nature 321, 607–610 (1986). https://doi.org/10.1038/321607a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/321607a0
This article is cited by
-
Skeletal muscle regeneration in Xenopus tadpoles and zebrafish larvae
BMC Developmental Biology (2012)
-
Heart of Newt: A Recipe for Regeneration
Journal of Cardiovascular Translational Research (2010)
-
Proteomic analysis of blastema formation in regenerating axolotl limbs
BMC Biology (2009)
-
Microarray and cDNA sequence analysis of transcription during nerve-dependent limb regeneration
BMC Biology (2009)
-
Reversibility of the mononucleate-to-multinucleate myogenic transition during amphibian limb regeneration
Eye (1994)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.