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Cyclic GMP-sensitive conductance in outer segment membrane of catfish cones

Abstract

A cyclic GMP-sensitive conductance has recently been observed with patch-clamp recording in excised inside-out patches of plasma membrane from frog and toad rod outer segments1,2. This conductance has properties suggesting that it is probably the light-sensitive conductance involved in visual transduction1,2. We now report a similar conductance in the outer segment membrane of catfish cones. Cyclic GMP showed positive cooperatively in opening this conductance, with a Hill coefficient of 1.6–3.0 and a half-saturating cGMP concentration of 35–70 µM. Cyclic AMP at 1 mM, or changing Ca concentration (in the presence of Mg), had little effect on the conductance. In physiological solutions the cGMP-induced current had a reversal potential near +10 mV; the current amplitude increased roughly exponentially with membrane potential in both depolarizing and hyperpolarizing directions. Our results suggest that cGMP is also the internal transmitter for phototransduction in cones.

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Haynes, L., Yau, KW. Cyclic GMP-sensitive conductance in outer segment membrane of catfish cones. Nature 317, 61–64 (1985). https://doi.org/10.1038/317061a0

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