Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Primary structure of the α-subunit of Torpedo californica (Na+ + K+)ATPase deduced from cDNA sequence

Nature volume 316, pages 733–736 (1985)Cite this article

Abstract

Sodium- and potassium-dependent ATPase [(Na+ + K+)ATPase], which is responsible for the active transport of Na+ and K+, is distributed universally among animal cell membranes and consists of two types of subunits, α and β (refs 1–4). The larger α-subunit with a relative molecular mass (Mr) of 84,000–120,000 is thought to have the catalytic role. We have now cloned and sequenced DNA complementary to the Torpedo californica electroplax messenger RNA encoding the α-subunit of (Na+ + K+)ATPase and have deduced the complete ammo-acid sequence of the polypeptide. Some structural features of the α-subunit molecule related to the function of this active-transport protein are discussed.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Kyte, J. Nature 292, 201–204 (1981).

    Article  ADS  CAS  PubMed  Google Scholar 

  2. Schuurmans-Stekhoven, F. & Bonting, S. L. Physiol. Rev. 61, 1–76 (1981).

    Article  CAS  PubMed  Google Scholar 

  3. Jørgensen, P. L. Biochim. biophys. Acta 694, 27–68 (1982).

    Article  PubMed  Google Scholar 

  4. Post, R. L. Curr. Top. Membr. Transp. 19, 53–65 (1983).

    Article  CAS  Google Scholar 

  5. Blobel, G. & Dobberstein, B. J. Cell Biol. 67, 852–862 (1975).

    Article  CAS  PubMed  Google Scholar 

  6. Cantley, L. C. Curr. Top. Bioenerg. 11, 201–237 (1981).

    Article  CAS  Google Scholar 

  7. Collins, J. H. & Zot, A. S. IRCS Med. Sci. Libr. Compend. 11, 799 (1983).

    CAS  Google Scholar 

  8. Hopkins, B. E., Wagner, H. Jr & Smith, T. W. J. biol Chem. 251, 4365–4371 (1976).

    CAS  PubMed  Google Scholar 

  9. Morohashi, M. & Kawamura, M. J. biol. Chem. 259, 14928–14934 (1984).

    CAS  PubMed  Google Scholar 

  10. Collins, J. H., Zot, A. S., Ball, W. J. Jr, Lane, L. K. & Schwartz, A. Biochim. biophys. Acta 742, 358–365 (1983).

    Article  CAS  PubMed  Google Scholar 

  11. Marshall, R. D. Biochem. Soc. Symp. 40, 17–26 (1974).

    CAS  Google Scholar 

  12. Kyte, J. & Doolittle, R. F. J. molec. Biol. 157, 105–132 (1982).

    Article  CAS  PubMed  Google Scholar 

  13. Chou, P. Y. & Fasman, G. D. A. Rev. Biochem. 47, 251–276 (1978).

    Article  CAS  Google Scholar 

  14. Post, R. L., Hegyvary, C. & Kume, S. J. J. biol. Chem. 247, 6530–6540(1972).

    CAS  PubMed  Google Scholar 

  15. Glynn, I. M. & Richards, D. E. Curr. Top. Membr. Transp. 19, 625–638 (1983).

    Article  CAS  Google Scholar 

  16. Walderhaug, M. O., Post, R. L., Saccomani, G., Leonard, R. T. & Briskin, D. P. J. biol. Chem. 260 3852–3859 (1985).

    CAS  PubMed  Google Scholar 

  17. Farley, R. A., Trant, C. M., Carilli, C. T., Hawke, D. & Shively, J. E. J. biol. Chem. 259, 9532–9535 (1984).

    CAS  PubMed  Google Scholar 

  18. Kirley, T. L., Wallick, E. T. & Lane, L. K. Biochem. biophys. Res. Commun. 125, 767–773 (1984).

    Article  CAS  PubMed  Google Scholar 

  19. Jørgensen, P. L., Karlish, S. J. D. & Gitler, C. J. biol. Chem. 257, 7435–7442 (1982).

    PubMed  Google Scholar 

  20. Repke, K. R. H., Kott, M. & Vogel, F. Biomed. biochim. Acta 42, 825–838 (1983).

    CAS  PubMed  Google Scholar 

  21. Nicholas, R. A. Biochemistry 23, 888–898 (1984).

    Article  CAS  PubMed  Google Scholar 

  22. O'Connell, M. A. Biochemistry 21, 5984–5991 (1982).

    Article  CAS  PubMed  Google Scholar 

  23. Sharkey, R. Biochim. biophys. Acta 730, 327–341 (1983).

    Article  CAS  PubMed  Google Scholar 

  24. Allen, G., Trinnaman, B. J. & Green, N. M. Biochem. J. 187, 591–616 (1980).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  25. Hesse, J. E. et al. Proc. natn. Acad. Sci. U.S.A. 81, 4746–4750 (1984).

    Article  ADS  CAS  Google Scholar 

  26. Hewick, R. M., Hunkapiller, M. W., Hood, L. E. & Dreyer, W. J. J. biol. Chem. 256, 7990–7997 (1981).

    CAS  PubMed  Google Scholar 

  27. Jørgensen, P. L. Biochim. biophys. Acta 356, 36–52 (1974).

    Article  PubMed  Google Scholar 

  28. Marshall, P. J. & Hokin, L. E. Biochem. biophys. Res. Commun. 87, 476–482 (1979).

    Article  CAS  PubMed  Google Scholar 

  29. Omori, K., Takemura, S., Omori, K., Mega, T. & Tashiro, Y. J. Biochem. 94, 1857–1866 (1983).

    Article  CAS  PubMed  Google Scholar 

  30. Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).

    Article  CAS  PubMed  Google Scholar 

  31. Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).

    Article  ADS  CAS  PubMed  Google Scholar 

  32. Okayama, H. & Berg, P. Molec. cell. Biol. 2, 161–170 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  33. Noda, M. et al. Nature 299, 793–797 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  34. Noda, M. et al. Nature 295, 202–206 (1982).

    Article  ADS  CAS  PubMed  Google Scholar 

  35. Ito, H., Ike, Y., Ikuta, S. & Itakura, K. Nucleic Acids Res. 10, 1755–1769 (1982).

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Weinstock, R., Sweet, R., Weiss, M., Cedar, H. & Axel, R. Proc. natn. Acad. Sci. U.S.A. 75, 1299–1303 (1978).

    Article  ADS  CAS  Google Scholar 

  37. Dayhoff, M. O., Schwartz, R. M. & Orcutt, B. C. in Atlas of Protein Sequence and Structure Vol. 5, Suppl. 3 (ed. Dayhoff, M. O.) 345–352 (National Biomedical Research Foundation, Silver Spring, Maryland, 1978).

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biology, Jichi Medical School, Yakushiji, Tochigi-ken, 329-04, Japan

    Kiyoshi Kawakami, Toshiko Ohta & Kei Nagano

  2. Department of Pharmacology, Jichi Medical School, Yakushiji, Tochigi-ken, 329-04, Japan

    Hiroshi Nojima

  3. Departments of Medical Chemistry and Molecular Genetics, Kyoto University Faculty of Medicine, Kyoto, 606, Japan

    Kiyoshi Kawakami, Shunsuke Noguchi, Masaharu Noda, Hideo Takahashi & Shosaku Numa

  4. Department of Biology, Chiba University Faculty of Science, Chiba, 280, Japan

    Shunsuke Noguchi & Masaru Kawamura

  5. Pharmaceutical Institute, Keio University School of Medicine, Tokyo, 160, Japan

    Tadaaki Hirose & Seiichi Inayama

  6. Department of Biology, Kyushu University Faculty of Science, Fukuoka, 812, Japan

    Hidenori Hayashida & Takashi Miyata

Authors
  1. Kiyoshi Kawakami
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shunsuke Noguchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Masaharu Noda
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hideo Takahashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Toshiko Ohta
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Masaru Kawamura
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Hiroshi Nojima
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Kei Nagano
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Tadaaki Hirose
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Seiichi Inayama
    View author publications

    You can also search for this author in PubMed Google Scholar

  11. Hidenori Hayashida
    View author publications

    You can also search for this author in PubMed Google Scholar

  12. Takashi Miyata
    View author publications

    You can also search for this author in PubMed Google Scholar

  13. Shosaku Numa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kawakami, K., Noguchi, S., Noda, M. et al. Primary structure of the α-subunit of Torpedo californica (Na+ + K+)ATPase deduced from cDNA sequence. Nature 316, 733–736 (1985). https://doi.org/10.1038/316733a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/316733a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing