Abstract
Haemophilia B, or Christmas disease, is an inherited X-chromosome-linked bleeding disorder caused by a defect in clotting factor IX and occurs in about 1 in 30,000 males in the United Kingdom1. Injection of factor IX concentrate obtained from blood donors allows most patients to be successfully managed. However, because of impurities in the factor IX concentrate presently in use, this treatment involves some risk of infection by blood-borne viruses such as non-A, non-B hepatitis and the virus causing acquired immune deficiency syndrome (AIDS)2. Because of the recent concern about the increasing incidence of AIDS amongst haemophiliacs, a factor IX preparation derived from a source other than blood is desirable. Here, we report that after introduction of human factor IX DNA clones3 into a rat hepatoma cell line using recombinant DNA methods, we were able to isolate small amounts of biologically active human factor IX.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
McKee, P. A. in The Metabolic Basis of Inherited Disease 5th edn (eds Stanbury, J. B., Wyngaarden, J. B., Fredrickson, D. S., Goldstein, J. L. & Brown, M. S.) 1531–1560 (McGraw-Hill, New York, 1983).
Lancet ii, 1433–1435 (1984).
Anson, D. S. et al. EMBO J. 3, 1053–1060 (1984).
Jackson, C. M. & Nemerson, Y. A. Rev. Biochem. 49, 765–811 (1980).
Suttie, J. W. CRC Crit. Rev. Biochem. 8, 191–223 (1980).
Chavin, S. I. & Weidner, S. M. J. biol. Chem. 259, 3387–3390 (1984).
Fernlund, P. & Stenflo, J. J. biol. Chem. 258, 12509–12512 (1983).
Munns, T. W., Johnston, M. F. M., Liszewski, M. K. & Olsen, R. E. Proc. natn. Acad. Sci. U.S.A. 73, 2803–2809 (1976).
Colbere-Garapin, F., Horodniceanu, F., Kourilsky, P. & Garapin, A.-C. J. molec. Biol. 150, 1–14 (1981).
Chu, G. & Sharp, P. A. Gene 13, 197–202 (1981).
Davies, J. & Jimenez, A. Am. J. trop. Med. Hyg. 29(5) Suppl., 1089–1092 (1980).
Fujikawa, K. & Davie, E. W. Meth. Enzym. 45, 77 (1976).
Austen, D. E. G. & Rhymes, I. L. A Laboratory Manual of Blood Coagulation (Blackwell Scientific, 1975).
Yoshioka, A., Giddings, J. C., Thomas, J. E., Fujimura, Y. & Bloom, A. R. Br. J. Haemat. 59, 265–275 (1985).
Wood, W. I. et al. Nature 312, 330–336 (1984).
Radcliffe, R. & Nemerson, Y. Meth. Enzym. 45, 51 (1976).
Krieg, P. et al. J. molec. Biol. 180, 615–643 (1984).
Grosveld, F. G. et al. Nucleic Acids Res. 10, 6715–6732 (1982).
Toole, J. J. et al. Nature 312, 342–347 (1984).
Lang, J. C., Wilkie, N. M. & Spandidos, D. A. J. gen. Virol. 64, 2679–2696 (1983).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Anson, D., Austen, D. & Brownlee, G. Expression of active human clotting factor IX from recombinant DNA clones in mammalian cells. Nature 315, 683–685 (1985). https://doi.org/10.1038/315683a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/315683a0
This article is cited by
-
Prevention and Management of Bleeding Episodes in Children with Hemophilia
Pediatric Drugs (2018)
-
Heterochromatin Protein 1 Deleted Chromo Domain Decreases Gene Silencing of Transgene in Mouse
Biotechnology Letters (2006)
-
Drosophila cells can be grown to high cell densities in a bioreactor
Biotechnology Techniques (1996)
-
Recombinant human protein C, protein S and thrombomodulin as antithrombotics
Perspectives in Drug Discovery and Design (1994)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.