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Increased MHC H–2K gene transcription in cultured mouse embryo cells after adenovirus infection

Nature volume 315, pages 579–581 (1985)Cite this article

Abstract

The class I major histocompatibility complex (MHC) antigens are highly polymorphic1 cell-surface proteins2 whose expression is essential for the cellular immune response against virus-infected, abnormal and foreign cells3,4. Transformation of primary rat cell cultures by the oncogenic adenovirus 12 (Ad 12) results in suppression of the transplantation antigens5, thus enabling the transformed cells to escape the immune response and efficiently form tumours in vivo6. In contrast, transformation of the same cells with the non-oncogenic adenovirus 5 (Ad5) does not suppress the transplantation antigens5 and, consequently, they elicit an effective (MHC-restricted) immune response6. Here, however, we show that infection of mouse embryo cells with both viruses initially increases the level of transcripts from the H–2Kb transplantation antigen gene. Both the adenovirus E1a (12S RNA) and E1b genes are required for activation of the H–2K gene and measurement of the relative rate of transcription indicates that the increase in the level of H–2K messenger RNA following infection is at least in part due to a gene-specific transcriptional activation. The newly transcribed H–2Kb mRNA is then properly transported to the cytoplasm.

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Authors and Affiliations

  1. Laboratory of Gene Structure and Expression, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK

    Arnon Rosenthal, Stephanie Wright & Frank Grosveld

  2. Division of Virology, National Institute for Medical Research, The Ridgeway, Mill Hill, London, NW7 1AA, UK

    Kristina Quade

  3. Department of Molecular Biology, Hadassah Medical School, The Hebrew University, Jerusalem, Israel

    Arnon Rosenthal & Howard Cedart

  4. Department of Cancer Studies, CRC Laboratory, University of Birmingham, Birmingham, B15 2TJ, UK

    Phillip Gallimore

Authors
  1. Arnon Rosenthal
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  2. Stephanie Wright
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  4. Phillip Gallimore
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  5. Howard Cedart
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  6. Frank Grosveld
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Rosenthal, A., Wright, S., Quade, K. et al. Increased MHC H–2K gene transcription in cultured mouse embryo cells after adenovirus infection. Nature 315, 579–581 (1985). https://doi.org/10.1038/315579a0

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