Abstract
In proliferating B lymphocytes, somatic mutation of rearranged antibody variable (V)-region genes occurs at high frequency and may have a key role in the selection of these cells1–3. It is of interest in this context to learn in which way single mutations can affect antigen binding and/or idiotypic specificity of an antibody. Previous investigations have analysed spontaneous mutants of myeloma and hybridoma cells in which the mutation affected the antigen-binding specificity of the antibody4–8. Here we describe an antibody mutant that has fully retained antigen-binding specificity but has lost or drastically changed all V-region antigenic determinants (idiotopes) of the wild type as defined by monoclonal anti-idiotope antibodies. The mutant phenotype is generated by a glycine to arginine exchange in the middle of the diversity (D) element, at position 103 of the heavy chain.
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Radbruch, A., Zaiss, S., Kappen, C. et al. Drastic change in idiotypic but not antigen-binding specificity of an antibody by a single amino-acid substitution. Nature 315, 506–508 (1985). https://doi.org/10.1038/315506a0
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DOI: https://doi.org/10.1038/315506a0
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