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Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase

Abstract

Chimaeric genes can be constructed which fuse the transit peptide of a small subunit of the chloroplast-located ribulose l,5-bisphosphate carboxylase with a bacterial protein. The fusion protein is translocated into chloroplasts and cleaved in a similar way to the small subunit polypeptide precursor.

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References

  1. Ellis, R. J. A. Rev. Pl. Physiol. 32, 111–137 (1981).

    CAS  Article  Google Scholar 

  2. Gillham, N., Boynton, J. & Chua, N.-H. Curr. Topics Bioengng 8, 211–260 (1978).

    CAS  Google Scholar 

  3. Gray, J. C. & Kekwick, R. G. O. Eur. J. Biochem. 44, 491–503 (1974).

    CAS  Article  Google Scholar 

  4. Cashmore, A. J. biol. Chem. 251, 2848–2853 (1976).

    CAS  PubMed  Google Scholar 

  5. Apel, K. & Kloppstech, K. Eur. J. Biochem. 85, 581–588 (1978).

    CAS  Article  Google Scholar 

  6. Highfield, P. E. & Ellis, R. J. Nature 271, 420–424 (1978).

    ADS  CAS  Article  Google Scholar 

  7. Dobberstein, B., Blobel, G. & Chua, N.-H. Proc. natn. Acad. Sci. U.S.A. 74, 1082–1085 (1977).

    ADS  CAS  Article  Google Scholar 

  8. Schmidt, G. W., Bartlett, S. G., Grossman, A. R., Cashmore, A. R. & Chua, N.-H. J. Cell Biol. 91, 468–478 (1981).

    CAS  Article  Google Scholar 

  9. Cashmore, A. R., Broadhurst, M. K. & Gray, R. E. Proc. natn. Acad. Sci. U.S.A. 75, 655–659 (1978).

    ADS  CAS  Article  Google Scholar 

  10. Grossman, A. R., Bartlett, S. G. & Chua, N.-H. Nature 285, 625–628 (1980).

    ADS  CAS  Article  Google Scholar 

  11. Chua, N.-H. & Schmidt, G. W. J. Cell Biol 81, 461–483 (1979).

    CAS  Article  Google Scholar 

  12. Chua, N.-H. & Schmidt, G. W. Proc. natn. Acad. Sci. U.S.A. 75, 6110–6114 (1978).

    ADS  CAS  Article  Google Scholar 

  13. Ellis, R. J., Smith, S. M. & Barraclough, R. in Genome Organization and Expression in Plants (ed. Leaver, C. J.) 321–335 (Plenum, New York, 1980).

    Book  Google Scholar 

  14. Schmidt, G. W., Bartlett, S. G., Grossman, A. R., Cashmore, A. R. & Chua, N.-H. in Genome Organization and Expression in Plants (ed. Leaver, C. J.) 337–351 (Plenum, New York, 1980).

    Book  Google Scholar 

  15. Grossman, A. R., Bartlett, S. G., Schmidt, G. W., Mullet, J. E. & Chua, N.-H. J. biol. Chem. 257, 1558–1563 (1982).

    CAS  PubMed  Google Scholar 

  16. Grossman, A. R., Bartlett, S. G., Schmidt, G. W. & Chua, N.-H. Ann. N.Y. Acad. Sci. 343, 266–274 (1980).

    ADS  CAS  Article  Google Scholar 

  17. Chua, N.-H. & Schmidt, G. W. in Photosynthetic Carbon Assimilation (eds Siegelman, H. W. & Hind, G.) 325–347 (Plenum, New York, 1978).

    Book  Google Scholar 

  18. Robinson, C. & Ellis, J. R. Eur. J. Biochem. 142, 343–346 (1984).

    CAS  Article  Google Scholar 

  19. Schmidt, G. W. et al. J. Cell Biol. 83, 615–622 (1979).

    CAS  Article  Google Scholar 

  20. Broglie, R., Coruzzi, G., Lamppa, G., Keith, B. & Chua, N.-H. Bio/technology 1, 55–61 (1983).

    Google Scholar 

  21. Timko, M. P. & Cashmore, A. R. in Plant Molecular Biology (ed. Goldberg, R. B.) 403–412 (Liss, New York, 1983).

    Google Scholar 

  22. Coruzzi, G., Broglie, R., Lamppa, G. & Chua, N.-H. in Structure and Function of Plant Genomes (eds Ciferri, O. & Dure, L. III) 47–59 (Plenum, New York, 1984).

    Google Scholar 

  23. Broglie, R. et al. Science 224, 838–843 (1984).

    ADS  CAS  Article  Google Scholar 

  24. Mishkind, N. L., Wessler, S. S. & Schmidt, G. W. J. Cell Biol. (in the press).

  25. Silhavy, T. J. et al. Proc. natn. Acad. Sci U.S.A. 74, 5411–5415 (1977).

    ADS  CAS  Article  Google Scholar 

  26. Wolfe, P. B. & Wickner, W. Cell 36, 1067–1072 (1984).

    CAS  Article  Google Scholar 

  27. Gething, M.-J. & Sambrook, J. Nature 300, 598–603 (1982).

    ADS  CAS  Article  Google Scholar 

  28. Sabatini, D. D., Kreibich, G., Morimoto, T. & Adesnik, M. J. Cell Biol. 92, 1–22 (1982).

    CAS  Article  Google Scholar 

  29. Emr, S. D. & Silvahy, T. J. Cell Biol. 95, 689–696 (1982).

    CAS  Article  Google Scholar 

  30. Benson, S. A. & Silvahy, T. Cell 32, 1325–1335 (1983).

    CAS  Article  Google Scholar 

  31. Guarente, L. & Ptashne, M. Proc. natn. Acad. Sci U.S.A 78, 2460–2464 (1981).

    Article  Google Scholar 

  32. Hall, M. N., Hereford, L. & Herskowitz, I. Cell 36, 1057–1065 (1984).

    CAS  Article  Google Scholar 

  33. Lignappa, V. R., Chaidez, J., Yost, C. S. & Hedpeth, J. Proc. natn. Acad. Sci. U.S.A. 81, 456–460 (1984).

    ADS  Article  Google Scholar 

  34. Douglas, M. G., Geller, B. L. & Emr, S. S. Proc. natn. Acad. Sci. U.S.A. 81, 3983–3987 (1984).

    ADS  CAS  Article  Google Scholar 

  35. Cashmore, A. R. in Genetic Engineering of Plants—An Agricultural Perspective (eds Kosuge, T., Meredith, C. P. & Hollaender, A.) 29–38 (Plenum, New York, 1983).

    Book  Google Scholar 

  36. Herrera-Estrella, L. et al. Nature 310, 115–120 (1984).

    ADS  CAS  Article  Google Scholar 

  37. Herrera-Estrella, L., Depicker, A., Van Montagu, M. & Schell, J. Nature 303, 209–213 (1983).

    ADS  CAS  Article  Google Scholar 

  38. Fraley, R. T. et al. Proc. natn. Acad. Sci. U.S.A. 80, 4803–4807 (1983).

    ADS  CAS  Article  Google Scholar 

  39. Horsch, R. B. et al. Science 223, 496–498 (1984).

    ADS  CAS  Article  Google Scholar 

  40. Bevan, M. W., Flavell, R. B. & Chilton, M.-D. Nature 304, 184–187 (1983).

    ADS  CAS  Article  Google Scholar 

  41. Murai, N. et al. Science 222, 476–482 (1983).

    ADS  CAS  Article  Google Scholar 

  42. Herrera-Estrella, L. et al. EMBO J. 2, 987–995 (1983).

    CAS  Article  Google Scholar 

  43. Caplan, A. et al. Science 222, 815–821 (1983).

    ADS  CAS  Article  Google Scholar 

  44. De Block, M. et al. EMBO J. 3, 1681–1689 (1984).

    CAS  Article  Google Scholar 

  45. Reiss, B. thesis, Univ. Heidelberg (1982).

  46. Reiss, B., Sprengel, R., Will, H. & Schaller, H. Gene 30, 217–223 (1984).

    Article  Google Scholar 

  47. Zambryski, P., Herrera-Estrella, L., De Block, M., Van Montagu, M. & Schell, J. in Genetic Engineering, Principles and Methods Vol. 6 (eds Setlow, J. & Hollaender, A.) 253–278 (Plenum, New York, 1984).

    Google Scholar 

  48. Van Haute, E. et al. EMBO J. 2, 411–418 (1983).

    CAS  Article  Google Scholar 

  49. Southern, E. M. J. molec. Biol. 98, 503–518 (1975).

    CAS  Article  Google Scholar 

  50. Murashige, T. & Skoog, F. Physiologia Pl. 15, 473–497 (1962).

    CAS  Article  Google Scholar 

  51. Bartlett, S. G., Grossman, A. R. & Chua, N.-H. in Methods in Chloroplast Molecular Biology (ed. Edelman, M.) 1081–1091 (Elsevier, Amsterdam, 1982).

    Google Scholar 

  52. Lathe, R., Balland, A., Kohli, V. & Lecocq, J.-P. Gene 20, 187–195 (1982).

    CAS  Article  Google Scholar 

  53. De Greve, H. et al. J. Molec. appl. Genet. 1, 499–512 (1982).

    CAS  Google Scholar 

  54. Vieira, J. & Messing, J. Gene 19, 259–268 (1982).

    CAS  Article  Google Scholar 

  55. Dhaese, P. et al. EMBO J. 2, 419–426 (1983).

    CAS  Article  Google Scholar 

  56. Dellaporta, S. L., Wood, J. & Hicks, J. B. Pl. molec. Biol. Rep. 1, 19–21 (1983).

    CAS  Article  Google Scholar 

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Van den Broeck, G., Timko, M., Kausch, A. et al. Targeting of a foreign protein to chloroplasts by fusion to the transit peptide from the small subunit of ribulose 1,5-bisphosphate carboxylase. Nature 313, 358–363 (1985). https://doi.org/10.1038/313358a0

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  • DOI: https://doi.org/10.1038/313358a0

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