Abstract
The HLA class II antigens are a highly polymorphic family of dimeric cell-surface glycoproteins, expressed predominantly on the surface of immunocompetent cells. They are intimately involved with the induction of the T-cell response to extrinsic antigen1–4 and are important predisposing factors for a wide spectrum of autoimmune diseases5. We describe here the expression of a class II product from the HLA-DP (new WHO nomenclature, formerly SB) subregion after transfer of cloned genes into mouse fibroblasts. The transfected DP antigen is recognized by several HLA class II monoclonal antibodies and, though present in a mouse cell background, is able to function in the presentation of influenza antigen to cloned DP-restricted human T lymphocytes.
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References
Klein, J. & Nagy, Z. A. Adv. Cancer Res. 37, 233–317 (1982).
Bodmer, W. F. in Mammalian Genetics and Cancer (ed. Russel, E.) 213–240 (Liss, New York, 1981).
Schwartz, R. H. in Ia Antigens (eds Ferrone, S. & David, C. S. ) 161–218 (CRC Press, Boca Raton, 1982).
Kaufman, J. F., Auffray, C., Korman, A. J., Shackelford, D. A. & Strominger, J. Cell 36, 1–13 (1984).
Svejgaard, A., Platz, P. & Ryder, L. P. Immun. Rev. 70, 193–218 (1983).
Spielman, R., Lee, J. S., Bodmer, W. F., Bodmer, J. G. & Trowsdale, J. Proc. natn. Acad. Sci. U.S.A. 81, 3461–3465 (1984).
Shaw, S., Pollack, M. S., Payne, S. M. & Johnson, A. H. Hum. Immun., 1, 177–185 (1980).
Trowsdale, J. et al. Cell 38, 241–249 (1984).
Rabourdin-Combe, C. & Mach, B. Nature 303, 670–674 (1983).
Germain, R. N., Norcross, M. A. & Margulies, D. H. Nature 306, 190–194 (1983).
Foisom, V. et al. Proc. natn. Acad. Sci. U.S.A. 81, 2045–2049 (1984).
Malissen, B. et al. Cell 36, 319–327 (1984).
Watson, A. J., DeMars, R., Trowbridge, I. S. & Bach, F. C. Nature 304, 358–361 (1983).
Shaw, S., Ziegler, A. & DeMars, R. Hum. Immun. (in the press).
Bodmer, J. G. & Bodmer, W. F. Br. med. Bull. (in the press).
Crumpton, M. J. et al. in Histocompatibility Testing 1984 (eds Albert, E. & Mayr, W.) (Springer, New York, in the press).
Rudd, C. E., Bodmer, J. G., Bodmer, W. F. & Crumpton, M. J. in Histocompatibility Testing 1984 (eds Albert, E. & Mayr, W.) (Springer, New York, in the press).
de Kretser, T. A., Crumpton, M. J., Bodmer, J. G. & Bodmer, W. F. Molec. biol. Med. 1, 59–76 (1983).
Eckels, D. D. et al. Nature 301, 716–718 (1983).
Lamb, J. R., Eckels, D. D., Phelan, M. & Woody, J. N. J. Immun. 128, 1428–1432 (1982).
Green, N. et al. Cell 28, 477–488 (1982).
Londei, M., Lamb, J. R., Bottazzo, G. F. & Feldmann, M. Nature (in the press).
Meuer, S. C. et al. Science 218, 471–473 (1982).
Biddison, W., Rao, P. E., Talle, M. A., Goldstein, G. & Shaw, S. J. Immun. 131, 152–157 (1983).
Springer, T. A. et al. Immun. Rev. 63, 171–195 (1982).
Trowsdale, J. et al. Proc. natn. Acad. Sci. U.S.A. 80, 1972–1976 (1983).
Wigler, M., Pellicor, A., Silverstein, S. & Axel, R. Cell 14, 725–731 (1978).
Gorman, C. M., Howard, B. H. & Reeves, R. Nucleic Acids Res. 11, 7631–7649 (1983).
Gillies, S. D., Morrison, S. L., Oi, V. T. & Tonegawa, S. Cell 33, 717–718 (1983).
O'Farrell, P. L., Goodman, H. J. M. & O'Farrell, P. H. Cell 12, 1133–1142 (1977).
Rudd, C. E., Bodmer, J. G., Bodmer, W. F. & Crumpton, M.J. J. biol. Chem. (in the press).
Hubbard, A. L. & Cohn, Z. A. Biochemical Analysis of Membranes (ed. Maddy, A. H.) 427–501 (Chapman & Hall, London, 1976).
Lamb, J. R. & Feldmann, M. Nature 308, 72–74 (1984).
Lamb, J. R., Eckels, D. D., Lake, P., Woody, J. N. & Green, N. Nature 300, 66–69 (1982).
Shaw, S. & De Mars, R. Disease Markers 2, 183–195 (1984).
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Austin, P., Trowsdale, J., Rudd, C. et al. Functional expression of HLA-DP genes transfected into mouse fibroblasts. Nature 313, 61–64 (1985). https://doi.org/10.1038/313061a0
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DOI: https://doi.org/10.1038/313061a0
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