Abstract
The sequence AAUAAA, found 11–30 base pairs (bp) upstream of the poly(A) site of most non-histone eukaryotic messenger RNAs (mRNAs)1 forms an essential part of the recognition site for 3′-end processing of the primary transcript2–4. However, the sequence AATAAA is found in transcribed regions of genes5–8 and is differentially utilized in genes containing multiple copies of the sequence within the 3′-noncoding region8–18, suggesting that the hexanucleotide alone does not comprise a complete recognition site. Therefore, it seems likely that additional sequences are required to form a complete recognition site for 3′-end formation. We have investigated the sequence requirements for mRNA 3′-end formation using the rabbit β-globin gene as a model system. Here we demonstrate that an additional sequence 3′ to AAUAAA is required for the correct 3′-end formation of rabbit β-globin mRNA.
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References
Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).
Fitzgerald, M. & Shenk, T. Cell 24, 251–260 (1981).
Montell, C., Fisher, E. F., Caruthers, M. H. & Berk, A. J. Nature 305, 600–605 (1983).
Higgs, D. R. et al. Nature 306, 398–400 (1983).
Fiers, W. et al. Nature 273, 113–120 (1980).
Reddy, V. B., Ghosh, P. K., Lebowitz, P., Piatek, M. & Weissman, S. J. Virol. 30, 279–296 (1979).
Perricaudet, M., le Moullec, J.-M., Tiollais, P. & Petterson, U. Nature 288, 174–176 (1980).
Aho, S., Tate, U. & Boedtker, H. Nucleic Acids Res. 11, 5443–5450 (1983).
Alt, F. W. et al. Cell 20, 293–301 (1980).
Tosi, M., Young, R. A., Hagenbuchle, O. & Schibler, U. Nucleic Acids Res. 9, 2313–2323 (1981).
Setzer, D. R., McGrogan, M., Nunberg, J. H. & Schimke, R. T. Cell 22, 361–370 (1980).
Sharp, P. A., Manley, J., Fire, A. & Gefter, M. Ann. N. Y. Acad. Sci. 354, 1–15 (1980).
Early, P. et al. Cell 20, 313–319 (1980).
Rosenfeld, M. G. et al. Nature 304, 128–135 (1983).
Nevins, J. R. & Chen-Kiang, S. Adv. Virus Res. 26, 1–35 (1981).
Parnes, J. R., Robinson, R. R. & Seidman, J. G. Nature 302, 449–452 (1983).
Henikoff, S., Sloan, J. S. & Kelly, J. D. Cell 34, 405–414 (1983).
Capetanaki, Y. G., Ngai, J., Flytzanis, C. N. & Lazarides, E. Cell 35, 411–420 (1983).
Grosveld, G. C., de Boer, E., Shewmaker, C. K. & Flavell, R. A. Nature 295, 120–126 (1982).
Chu, G. & Sharp, P. A. Gene 13, 197–202 (1981).
Berk, A. J. & Sharp, P. A. Cell 12, 721–732 (1977).
Weaver, R. F. & Weissman, C. Nucleic Acids Res. 6, 1175–1193 (1979).
Aviv, H. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 69, 1408–1412 (1972).
Taya, Y. et al. EMBO J. 1, 935–958 (1982).
Berget, S. M. Nature 309, 179–181 (1984).
McDevitt, M. A., Imperiale, M. J., Ali, H. & Nevins, J. R. Cell 37, 993–999 (1984).
Maniatis, T., Fritsch, E. F. & Sambrook, J. in Molecular Cloning (Cold Spring Harbor Laboratory, New York, 1982).
Maxam, A. M. & Gilbert, W. Meth. Enzym. 65, 499–560 (1980).
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Gil, A., Proudfoot, N. A sequence downstream of AAUAAA is required for rabbit β-globin mRNA 3′-end formation. Nature 312, 473–474 (1984). https://doi.org/10.1038/312473a0
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DOI: https://doi.org/10.1038/312473a0
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