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Molecular cloning of a human DNA repair gene

Nature volume 310, pages 425–429 (1984)Cite this article

Abstract

Cell strains derived from patients having a hereditary disorder associated with defects in repair of DNA damage such as xeroderma pigmentosum1,2 and mutants isolated from established rodent cell lines3,4 provide the tools for genetic and biochemical analysis of DNA repair pathways in mammalian cells. Complementation studies using these cells have illustrated the genetic and biochemical complexity of these pathways3,5,6. The precise nature of the genes and gene products involved in these mutants has not yet been resolved. Isolation of repair genes by recombinant DNA technology would open up new approaches to the elucidation of repair mechanisms in mammalian cells. Here we report the molecular cloning of a human repair gene (ERCC1) that complements the repair defect in a Chinese hamster ovary (CHO) mutant cell line.

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Author notes

  1. R. D. Wood

    Present address: Department of Molecular Biophysics and Biochemistry, Yale University, 260 Whitney Avenue, PO Box 6666, New Haven, Connecticut, 06511, USA

Authors and Affiliations

  1. Department of Cell Biology and Genetics, Erasmus University, Rotterdam, PO Box 1738, 3000 DR, Rotterdam, The Netherlands

    J. H. J. Hoeijmakers, M. van Duin, J. de Wit, H. Odijk & D. Bootsma

  2. Department of Medical Biochemistry, State University of Leiden, 2333 AL, Leiden, The Netherlands

    A. Pastink

  3. Lawrence Berkeley Laboratory, Biomedical Division and Graduate Group in Biophysics, University of California, Berkeley, California, 94720, USA

    R. D. Wood

Authors
  1. A. Westerveld
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  2. J. H. J. Hoeijmakers
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  3. M. van Duin
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  4. J. de Wit
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  5. H. Odijk
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  6. A. Pastink
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  7. R. D. Wood
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  8. D. Bootsma
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Westerveld, A., Hoeijmakers, J., van Duin, M. et al. Molecular cloning of a human DNA repair gene. Nature 310, 425–429 (1984). https://doi.org/10.1038/310425a0

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