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Staining of living presynaptic nerve terminals with selective fluorescent dyes

Abstract

Johnson et al. have recently shown that several positively charged, membrane-permeant fluorescent dyes can serve as vital stains for mitochondria in cultured cells1,2 (see also ref. 3). We report here that presynaptic nerve terminals, which are characteristically rich in mitochondria4,5, can also be vitally stained with such dyes, and that this staining offers a resolution of structural detail not available with previous methods for visualizing terminals in living tissues. The dyes have provided excellent, highly detailed fluorescence images of presynaptic motor nerve terminals at neuromuscular junctions in conventional live tissue preparations from mouse, frog and Drosophila. In addition, when tested at the frog neuromuscular junction, at least one of the dyes permitted visualization of terminals with little, if any, effect on synaptic transmission. Moreover, we have found that by using the dyes it is now also possible to see motor nerve terminals in situ in live animals.

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References

  1. Johnson, L. V., Walsh, M. L. & Ghen, L. B. Proc. natn. Acad. Sci. U.S.A. 77, 990–994 (1980).

    ADS  CAS  Article  Google Scholar 

  2. Johnson, L. V., Walsh, M. L., Bockus, B. J. & Chen, L. B. J. Cell Biol. 88, 526–535 (1981).

    CAS  Article  Google Scholar 

  3. Hetherington, D. C. Stain Technol. 11, 153–154 (1936).

    Article  Google Scholar 

  4. Gray, E. G. & Guillery, R. W. Int. Rev. Cytol. 19, 111–182 (1966).

    CAS  Article  Google Scholar 

  5. Peters, A., Palay, S. L. & Webester, H. deF. The Fine Structure of the Nervous System: The Neurons and Supporting Cells (W. B. Saunders, Philadelphia, 1976).

    Google Scholar 

  6. McMahan, U. J., Spitzer, N. C. & Peper, K. Proc. R. Soc. B181, 421–430 (1972).

    ADS  Google Scholar 

  7. McArdle, J. J. et al. J. Neurosci. Meth. 4, 109–115 (1981).

    CAS  Article  Google Scholar 

  8. Akert, K. & Sandri, C. Brain Res. 7, 286–295 (1968).

    CAS  Article  Google Scholar 

  9. Jan, L. Y. & Jan, Y. N. J. Physiol., Lond. 262, 189–214 (1976).

    CAS  Article  Google Scholar 

  10. Sims, P. J., Waggoner, A. S., Wang, C. H. & Hoffman, J. F. Biochemistry 13, 3315–3330 (1974).

    CAS  Article  Google Scholar 

  11. Waggoner, A. S., Wang, C. H. & Tolles, R. L. J. Membrane Biol. 33, 109–140 (1977).

    CAS  Article  Google Scholar 

  12. Alnaes, E. & Rahamimoff, R. J. Physiol., Lond. 248, 285–306 (1975).

    CAS  Article  Google Scholar 

  13. Waggoner, A. S. A. Rev. Biophys. Bioengng 8, 47–68 (1979).

    CAS  Article  Google Scholar 

  14. Freedman, J. C. & Laris, P. C. Int. Rev. Cytol. Suppl. 12, 177–246 (1981).

    CAS  PubMed  Google Scholar 

  15. McMahan, U. J. & Kuffller, S. W. Proc. R. Soc. B177, 485–508 (1971).

    ADS  CAS  Google Scholar 

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Yoshikami, D., Okun, L. Staining of living presynaptic nerve terminals with selective fluorescent dyes. Nature 310, 53–56 (1984). https://doi.org/10.1038/310053a0

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