Abstract
Mammalian atria contain potent natriuretic and diuretic substances1,2 which exist in high- and low-molecular-weight forms2,3 and which appear to be associated with atrium-specific granules4. The natriuretic effect of atrial extract is largely accountable for by its renal haemodynamic effects5–7; atrial extracts also antagonize hormone- and non-hormone-induced contraction of the isolated rabbit aorta8–10 and isolated rat kidney vasculature6. We have completely purified a low-molecular-weight natriuretic and vasoactive substance from rat atria and characterized it as a 24-amino acid peptide. Synthetic peptide, produced by solid-phase synthesis, mimics biological effects of crude atrial extract and purified peptide; its activity is enhanced by slow oxidation, suggesting a disulphide (Cys 4–Cys 20) configuration for the native peptide. If secreted into blood, this atrial natriuretic peptide (‘auriculin B‘) could be a novel peptide hormone of considerable importance to renal and cardiovascular homeostasis.
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References
deBold, A. J., Borenstein, H. R., Veress, A. T. & Sonnenberg, H. Life Sci. 28, 89–94 (1981).
deBold, A. J. Proc. Soc. exp. Biol. Med. 170, 133–138 (1982).
Trippodo, N. C., MacPhee, A. A., Cole, F. E. & Blakesley, H. L. Proc. Soc. exp. Biol. Med. 170, 502–508 (1982).
Garcia, R., Cantin, M., Thibault, G., Ong, H. & Genest, J. Experientia 38, 1071–1073 (1982).
Kleinert, H. D., Camargo, M. J. F., Sealey, J. E., Laragh, J. H. & Maack, T. Physiologist 24, 298 (1982).
Camargo, M. J. F. et al. Am. J. Physiol. 246, F447–F456 (1984).
Vaughan, E. D. Jr, et al. Surg. Forum 34, 690–692 (1983).
Deth, R. C. et al. Fedn Proc. 41, 983 (1982).
Currie, M. G. et al. Science 221, 71–73 (1983).
Kleinert, H. D. et al. Hypertension 6 Suppl. 1, 143–147 (1984).
Maack, T. Am. J. Physiol. 238, F71–F78 (1980).
deBold, A. J. & Flynn, T. G. Life Sci. 33, 297–302 (1983).
Grammer, R. T., Fukumi, H., Inagami, T. & Misono, K. S. Biochem. biophys. Res. Commun. 116, 696–703 (1983).
Currie, M. G. et al. Science 223, 67–69 (1984).
Borenstein, H. B., Cupples, W. A., Sonnenberg, H. & Veress, A. T. J. Physiol., Lond. 334, 133–140 (1983).
Jamieson, J. D. & Palade, G. E. J. Cell Biol. 23, 151–172 (1964).
Hunkapiller, M. W. & Hood, L. E. Meth. Enzym. 91, 486–492 (1983).
Lowry, O. H., Rosebrough, N. J., Farr, A. L. & Randall, R. J. J. biol. Chem. 193, 265–275 (1951).
Rivier, J. E. F. J. Am. chem. Soc. 96, 2986–2992 (1974).
Flynn, T. G., deBold, M. L. & deBold, A. J. Biochem. biophys. Res. Commun. 117, 859–865 (1983).
Kangawa, K. & Matsuo, H. Biochem. biophys. Res. Commun. 118, 131–139 (1984).
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Atlas, S., Kleinert, H., Camargo, M. et al. Purification, sequencing and synthesis of natriuretic and vasoactive rat atrial peptide. Nature 309, 717–719 (1984). https://doi.org/10.1038/309717a0
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DOI: https://doi.org/10.1038/309717a0
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