Abstract
We report here significant primary sequence homology among the predicted translational products of three genes: CDC4, CDC36 and ets. CDC4 and CDC36 are Saccharomyces cerevisiae cell division cycle genes, while ets is a transformation-specific sequence of avian erythroblastosis virus E26. The deduced primary structures of the three gene products were compared by computer to a large data base of known and predicted protein sequences1. The search revealed 22.0–25.5% identity over regions of 140–206 codons, respectively between the different pairwise combinations. For these particular sequences, these identity scores fall 3.4–4.0 standard deviations above the empirically-determined mean values of fortuitous similarity. S. cerevisiae calls require CDC36 and CDC4 in order to complete two early events in the cell cycle: execution of start (CDC36)2and spindle pole body separation (CDC4)3. In virus E26, the ets sequence is linked in frame with Δgag and mybEin the tripartite structure 5′-Δgag-mybE-ets-3′, comprising the E26 transforming oncogene4,5. The homologies described here suggest that the biochemical functions or regulation of the CDC4, CDC36 and ets products may be related.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Doolittle, R. F. Science 214, 149–159 (1981).
Reed, S. I. Genetics 95, 561–577 (1980).
Byers, B. & Goetsch, L. Cold Spring Harb. Symp. quant. Biol. 38, 123–131 (1974).
Nunn, M. F., Seeburg, P. H., Moscovici, C. & Duesberg, P. H. Nature 306, 391–395 (1983).
Leprince, D. et al. Nature 306, 395–397 (1983).
Pringle, J. R. & Hartwell, L. H. in The Molecular Biology of the Yeast Saccharomyces (eds Strathern, J. N., Jones, E. W. & Broach, J. R.) 79–142 (Cold Spring Harbor Laboratory, New York, 1981).
Hartwell, L. H., Culotti, J., Pringle, J. & Reid, B. Science 183, 46–51 (1974).
Hartwell, L. H. J. molec. Biol. 59, 183–194 (1971).
Hereford, L. M. & Hartwell, L. H. J. molec. Biol. 84, 445–461 (1974).
Hirschberg, J. & Simchen, G. Expl Cell Res. 105, 245–252 (1977).
Duesberg, P. H., Bister, K. & Moscovici, C. Proc. natn. Acad. Sci. U.S.A. 77, 5120–5124 (1980)
Bister, K. et al. Proc. natn. Acad. Sci. U.S.A. 79, 3677–3681 (1982).
Radke, K., Beug, H., Kornfeld, S. & Graf, T. Cell 31, 643–653 (1982).
Moscovici, C., Samurat, T., Gazzolo, L. & Moscovici, M. G. Virology 111, 765–768 (1981).
Lörincz, A. T. & Reed, S. I. Nature 307, 183–185 (1984).
Gallwitz, D., Donath, C. & Sander, C. Nature 306, 704–707 (1983).
DeFeo-Jones, D., Scolnick, E. M., Koller, R. & Dhar, R. Nature 306, 707–709 (1983).
Breter, H.-J., Ferguson, J., Peterson, T. A. & Reed, S. I. Molec. cell. Biol. 3, 881–891 (1983).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–574 (1977).
Messing, J., Crea, R. & Seeburg, P. A. Nucleic Acids Res. 9, 308–321 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Peterson, T., Yochem, J., Byers, B. et al. A relationship between the yeast cell cycle genes CDC4 and CDC36 and the ets sequence of oncogenic virus E26. Nature 309, 556–558 (1984). https://doi.org/10.1038/309556a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/309556a0
This article is cited by
-
The dTAFII80 subunit of Drosophila TFIID contains β-transducin repeats
Nature (1993)
-
SCM4, a gene that suppresses mutant cdc4 function in budding yeast
Molecular and General Genetics MGG (1992)
-
Suppression of temperature sensitive mutations in oncogene-related CDC genes in Saccharomyces cerevisiae by catabolite repression resistance and cytoplasmic petite mutations
Current Genetics (1985)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.