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Ha-ras oncogenes are activated by somatic alterations in human urinary tract tumours

Nature volume 309, pages 464–466 (1984)Cite this article

Abstract

DNA-mediated gene transfer (transfection) studies using NIH 3T3 cells as recipients have demonstrated the presence of transforming genes (oncogenes) in diverse human tumours (for reviews see refs 1, 2). A large proportion of oncogenes so far detected by DNA transfection are related to the Ha-ras onc gene of Harvey (and B ALB) murine sarcoma viruses (MSV)3–6, Ki-ras, the oncogene of Kirsten MSV3,7,8, and a third member of the ras gene family, N-ras (refs 8–12). Individual tumours of many different organs have been associated with the activation of members of the ras gene family. We now present the first systematic survey of human urinary tract tumours processed immediately after surgery, as well as normal tissues from the same patients, to detect the presence of such genes. We demonstrate activation of Ha-ras as an oncogene in around 10% of randomly selected urinary tract tumours as well as direct evidence that oncogene activation is the result of a somatic event which is selected for within the tumour cell population.

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Authors and Affiliations

  1. Laboratory of Cellular and Molecular Biology, National Cancer Institute, Building 37, Room 1A07, Bethesda, Maryland, 20205, USA

    Jun Fujita, Yasuhito Yuasa, Johng S. Rhim & Stuart A. Aaronson

  2. Department of Urology, Faculty of Medicine, Kyoto University, Kyoto, 606, Japan

    Osamu Yoshida

  3. Section of Serology and Immunology, Institute for Virus Research, Kyoto University, Kyoto, 606, Japan

    Masakazu Hatanaka

Authors
  1. Jun Fujita
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  2. Osamu Yoshida
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  3. Yasuhito Yuasa
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  4. Johng S. Rhim
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  5. Masakazu Hatanaka
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  6. Stuart A. Aaronson
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Fujita, J., Yoshida, O., Yuasa, Y. et al. Ha-ras oncogenes are activated by somatic alterations in human urinary tract tumours. Nature 309, 464–466 (1984). https://doi.org/10.1038/309464a0

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