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Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells

Abstract

The complete 1,210-amino acid sequence of the human epidermal growth factor (EGF) receptor precursor, deduced from cDNA clones derived from placental and A431 carcinoma cells, reveals close similarity between the entire predicted ν-erb-B mRNA oncogene product and the receptor transmembrane and cytoplasmic domains. A single transmembrane region of 23 amino acids separates the extracellular EGF binding and cytoplasmic domains. The receptor gene is amplified and apparently rearranged in A431 cells, generating a truncated 2.8-kilobase mRNA which encodes only the extracellular EGF binding domain.

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References

  1. 1

    Carpenter, G. Handbk exp. Pharmac. 57, 89–132 (1981).

    CAS  Article  Google Scholar 

  2. 2

    Guroff, G. (ed.) Growth and Maturation Factors Vol. 1 (Wiley, New York, 1983).

  3. 3

    Rozengurt, E. Molec. Biol. Med. 1, 169–181 (1983).

    CAS  PubMed  Google Scholar 

  4. 4

    Cohen, S., Carpenter, G. & King, L. Jr J. biol. Chem. 255, 4834–4842 (1980).

    CAS  Google Scholar 

  5. 5

    Ushiro, H. & Cohen, S. J. biol. Chem. 255, 8363–8365 (1980).

    CAS  Google Scholar 

  6. 6

    Carpenter, G. & Cohen, S. J. Cell Biol. 71, 154–171 (1976).

    Article  Google Scholar 

  7. 7

    Schlessinger, J. et al. Proc. natn. Acad. Sci. U.S.A. 75, 2659–2663 (1978).

    ADS  CAS  Article  Google Scholar 

  8. 8

    Pike, L. J. et al. J. biol. Chem. 258, 9282–9390 (1983).

    Google Scholar 

  9. 9

    Heldin, C.-H., Ek, B. & Ronnstrand, L. J. biol. Chem. 258, 10054–10061 (1983).

    CAS  PubMed  PubMed Central  Google Scholar 

  10. 10

    Kasuga, M. et al. Nature 298, 667–669 (1982).

    ADS  CAS  Article  Google Scholar 

  11. 11

    Roth, R. A. & Cassell, D. J. Science 219, 299–301 (1983).

    ADS  CAS  Article  Google Scholar 

  12. 12

    Jacobs, S. et al. J. biol. Chem. 258, 9581–9584 (1983).

    CAS  Google Scholar 

  13. 13

    Bishop, J. M. A. Rev. Biochem. 52, 301–354 (1983).

    CAS  Article  Google Scholar 

  14. 14

    Downward, J. et al. Nature 307, 521–527 (1984).

    ADS  CAS  Article  Google Scholar 

  15. 15

    Yamamoto, T. et al. Cell 35, 71–78 (1983).

    CAS  Article  Google Scholar 

  16. 16

    Fabricant, R. N., De Larco, J. E. & Todaro, G. J. Proc. natn. Acad. Sci. U.S.A. 74, 565–569 (1977).

    ADS  CAS  Article  Google Scholar 

  17. 17

    Wrann, M. M. & Fox, C. F. J. biol. Chem. 254, 8083–8086 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  18. 18

    Shimizu, N. & Kondo, I. Cytogenet. Cell Genet. 32, 316 (1982).

    Google Scholar 

  19. 19

    Gill, G. N. & Lazar, C. S. Nature 293, 305–307 (1981).

    ADS  CAS  Article  Google Scholar 

  20. 20

    Kawamoto, T. et al. Proc. natn. Acad. Sci. U.S.A. 80, 1337–1341 (1983).

    ADS  CAS  Article  Google Scholar 

  21. 21

    Huynh, T., Young, R. & Davis, R. in Practical Approaches in Biochemistry (ed. Grover, D.) (IRL, Oxford, 1984).

    Google Scholar 

  22. 22

    Mayes, E. L. V. & Waterfield, M. D. EMBO J. 3, 531–537 (1984).

    CAS  Article  Google Scholar 

  23. 23

    Crea, R. & Horn, T. Nucleic Acids Res. 8, 2331–2348 (1980).

    CAS  Article  Google Scholar 

  24. 24

    Ullrich, A., Berman, C. H., Dull, T. J., Gray, A. & Lee, J. M. EMBO J. 3, 361–364 (1984).

    CAS  Article  Google Scholar 

  25. 25

    Anderson, S. & Kingston, I. B. Proc. natn. Acad. Sci. U.S.A. 80, 6836–6842 (1983).

    ADS  Google Scholar 

  26. 26

    Jaye, M. et al. Nucleic Acids Res. 11, 2325–2335 (1983).

    CAS  Article  Google Scholar 

  27. 27

    Southern, E. J. molec. Biol. 98, 503–517 (1975).

    CAS  Article  Google Scholar 

  28. 28

    Sanger, F., Nicklen, S. & Coulson, A. R. Proc. natn. Acad. Sci. U.S.A. 74, 5463–5467 (1977).

    ADS  CAS  Article  Google Scholar 

  29. 29

    Messing, J. & Vieira, J. Gene 19, 269–276 (1982).

    CAS  Article  Google Scholar 

  30. 30

    Messing, J., Crea, R. & Seeburg, P. H. Nucleic Acids Res. 9, 309–321 (1981).

    CAS  Article  Google Scholar 

  31. 31

    Proudfoot, N. J. & Brownlee, G. G. Nature 263, 211–214 (1976).

    ADS  CAS  Article  Google Scholar 

  32. 32

    Privalsky, M. L., Ralston, R. & Bishop, J. M. Proc. natn. Acad. Sci. U.S.A. 81, 704–707 (1984).

    ADS  CAS  Article  Google Scholar 

  33. 33

    Levinson, A. D., Oppermann, H., Varmus, H. E. & Bishop, J. M. J. biol. Chem. 255, 11973–11980 (1980).

    CAS  Google Scholar 

  34. 34

    Erikson, R. L., Collett, M. S., Erikson, E. & Purchio, A. F. Proc. natn. Acad. Sci. U.S.A. 76, 6260–6264 (1979).

    ADS  CAS  Article  Google Scholar 

  35. 35

    Barker, W. C. & Dayhoff, M. D. Proc. natn. Acad. Sci. U.S.A. 79, 2836–2839 (1982).

    ADS  CAS  Article  Google Scholar 

  36. 36

    Lehrach, H., Diamond, D., Wozney, J. M. & Boedtker, H. Biochemistry 16, 4743–4751 (1977).

    CAS  Article  Google Scholar 

  37. 37

    Vennstrom, B. & Bishop, J. M. Cell 28, 135–143 (1982).

    CAS  Article  Google Scholar 

  38. 38

    Tosi, M., Young, R. A., Hagenbuchle, O. & Schibier, U. Nucleic Acids Res. 9, 2313–2323 (1981).

    CAS  Article  Google Scholar 

  39. 39

    Setzer, D. R., McGrogan, M., Nunberg, J. H. & Schimke, R. T. Cell 22, 361–370 (1980).

    CAS  Article  Google Scholar 

  40. 40

    McGrath, J. P. et al. Nature 304, 501–506 (1983).

    ADS  CAS  Article  Google Scholar 

  41. 41

    Amara, F. G. et al. Nature 298, 240–244 (1982).

    ADS  CAS  Article  Google Scholar 

  42. 42

    Waterfield, M. D. et al. J. cell. Biochem. 210, 149–161 (1982).

    Article  Google Scholar 

  43. 43

    Rose, J. K. & Bergmann, J. E. Cell 30, 753–757 (1982).

    CAS  Article  Google Scholar 

  44. 44

    Shimizu, N., Behzadian, M. A. & Shimizu, Y. Proc. natn. Acad. Sci. U.S.A. 77, 3600–3604 (1980).

    ADS  CAS  Article  Google Scholar 

  45. 45

    Davies, R. L., Grosse, V. A., Kucherlapati, R. & Bothwell, M. Proc. natn. Acad. Sci. U.S.A. 77, 4188–4192 (1980).

    ADS  CAS  Article  Google Scholar 

  46. 46

    Engelman, D. M., Henderson, R., Mchachlen, A. D. & Wallace, B. A. Proc. natn. Acad. Sci. U.S.A. 77, 2023–2027 (1980).

    ADS  CAS  Article  Google Scholar 

  47. 47

    Noda, M. et al. Nature 302, 528–532 (1983).

    ADS  CAS  Article  Google Scholar 

  48. 48

    Zidovetzki, R., Yarden, Y., Schlessinger, J. & Jovin, T. M. Proc. natn. Acad. Sci. U.S.A. 78, 6981–6985 (1981).

    ADS  CAS  Article  Google Scholar 

  49. 49

    Haigler, H. T., McKanna, J. A. & Cohen, S. J. Cell Biol. 81, 382–395 (1979).

    CAS  Article  Google Scholar 

  50. 50

    Schechter, Y. S., Hernaez, L., Schlessinger, J. & Cuatrecasas, P. Nature 278, 835–837 (1979).

    ADS  CAS  Article  Google Scholar 

  51. 51

    Schreiber, A. B. et al. J. biol. Chem. 258, 846–853 (1983).

    CAS  PubMed  Google Scholar 

  52. 52

    Schreiber, A. B. et al. Proc. natn. Acad. Sci. U.S.A. 78, 7535–7539 (1981).

    ADS  CAS  Article  Google Scholar 

  53. 53

    Parker, P. J. et al. J. biol. Chem. (in the press).

  54. 54

    Wahl, G. M., Vitto, L., Padgett, R. A. & Stark, G. R. Molec. cell. Biol. 2, 308–319 (1982).

    CAS  Article  Google Scholar 

  55. 55

    Wahl, G. M., Saint Vincent, B. R. & De Rose, M. L. Nature 307, 516–520 (1984).

    ADS  CAS  Article  Google Scholar 

  56. 56

    Barnes, D. W. J. Cell Biol. 93, 1–4 (1982).

    CAS  Article  Google Scholar 

  57. 57

    Grantham, R., Gautier, C., Gouy, M., Jacobzone, M. & Mercier, R. Nucleic Acids Res. 9, 43–74 (1981).

    Article  Google Scholar 

  58. 58

    Maniatis, T., Fritsch, E. F. & Sambrook, J. Molecular Cloning (Cold Spring Harbor Laboratory, New York, 1982).

    Google Scholar 

  59. 59

    Gray, A., Dull, T. J. & Ullrich, A. Nature 303, 722–725 (1983).

    ADS  CAS  Article  Google Scholar 

  60. 60

    Benton, W. D. & Davis, R. W. Science 196, 180–182 (1977).

    ADS  CAS  Article  Google Scholar 

  61. 61

    Smart, J. E. et al. Proc. natn. Acad. Sci. U.S.A. 78, 6013–6017 (1981).

    ADS  CAS  Article  Google Scholar 

  62. 62

    Taylor, J. M., Illmensee, R. & Summers, J. Biochim. biophys. Acta 4, 324–330 (1976).

    Article  Google Scholar 

  63. 63

    Weber, W. et al. Science 224, 294–297 (1984).

    ADS  CAS  Article  Google Scholar 

  64. 64

    Merlino, G. T. et al. Science 224, 417–419 (1984).

    ADS  CAS  Article  Google Scholar 

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Ullrich, A., Coussens, L., Hayflick, J. et al. Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 309, 418–425 (1984). https://doi.org/10.1038/309418a0

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