Abstract
Immune response genes1,2 of the murine major histocompatibility complex encode cell-surface glycoproteins that are expressed predominantly on B cells and macrophages and regulate immune responsiveness by restricting antigen recognition by T cells. The two classes of immune response molecule, termed I-A and I-E, are each comprised of two polymorphic chains (α and β), and nucleotide sequence analysis of genomic or cDNA clones has revealed that most of the amino acid differences between allelic I-A α or β chains occur in the first extracellular domain3,4. The mutant mouse strain B6.C-H-2bm12 (bm12), which differs from its parental strain C57BL/6 (B6) at the I-Aβ locus5–14, exhibits an immune response profile markedly different from that of B6. Here we present the nucleotide sequence of the mutant bm12 I-Aβ gene. Sequence comparison within the coding regions reveals three productive nucleotide differences between the I-Aβ genes of B6 and bm12 mice, all three differences occurring within a stretch of 14 nucleotides in the exon encoding the first extracellular domain. The clustered nature of the bm12 mutation, as well as the specific amino acid changes it engenders, suggest a possible mechanism for the generation of polymorphism in class II antigens.
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References
Uhr, J. W., Capra, J. D., Vitetta, E. S. & Cook, R. G. Science 206, 292–297 (1979).
Benacerraf, B. Science 212, 1229–1238 (1981).
Benoist, C. O., Mathis, D. J., Kanter, M. R., Williams, V. E. II & McDevitt, H. O. Cell 34, 169–177 (1983).
Choi, E., Mclntyre, K., Germain, R. N. & Seidman, J. G. Science 221, 283–286 (1983).
McKenzie, I. F. C. et al. J. exp. Med. 150, 1323–1338 (1979).
McKenzie, I. F. C., Sandrin, M. S., Morgan, G. M., Henning, M. M. & Melvold, R. W. Immunogenetics 11, 103–106 (1980).
Michaelides, M. et al. J. exp. Med. 153, 464–469 (1981).
Lin, C.-C. S., Rosenthal, A. S., Passmore, H. C. & Hansen, T. H. Proc. natn. Acad. Sci. U. S. A. 78, 6406–6410 (1981).
Krco, C. J., Kazim, A. L., Atassi, M. Z., Melvold, R. W. & David, C. S. J. Immunogenet. 8, 471–476 (1981).
Lei, H.-Y., Melvold, R. W., Miller, S. D. & Waltenbaugh, C. J. exp. Med. 156, 596–609 (1982).
Lafuse, W. P., McCormick, J. F., Melvold, R. W. & David, C. S. Transplantation 31, 434–438 (1981).
Hansen, T. H., Walsh, W. D., Ozato, K., Arn, J. S. & Sachs, D. H. J. Immun. 127, 2228–2231 (1981).
McKean, D. J., Melvold, R. W. & David, C. Immunogenetics 14, 41–51 (1981).
Lee, D. R., Hansen, T. H. & Cullen, S. E. J. Immun. 129, 245–251 (1982).
Larhammar, D. et al. Cell 34, 179–188 (1983).
Baltimore, D. Cell 24, 592–594 (1981).
Egel, R. Nature 290, 191–192 (1981).
Saito, H., Maki, R. A., Clayton, L. K. & Tonegawa, S. Proc. natn. Acad. Sci. U. S. A. 80, 5520–5524 (1983).
Kohn, H. I. et al. Immunogenetics 7, 279–294 (1978).
Nairn, R., Yamaga, K. & Nathenson, S. G. A. Rev. Genet. 14, 241–277 (1980).
Evans, G. A., Margulies, D. H., Camerini-Otero, R. D., Ozato, K. & Seidman, J. G. Proc. natn. Acad. Sci. U. S. A. 79, 1994–1998 (1982).
Pease, L. R., Schulze, D. H., Pfaffenbach, G. M. & Nathenson, S. G. Proc. natn. Acad. Sci. U. S. A. 80, 242–246 (1983).
Weiss, E. H. et al. Nature 301, 671–674 (1983).
Mellor, A. L., Weiss, E. H., Ramachandran, K. & Flavell, R. A. Nature 306, 792–795 (1983).
Klein, J. Science 203, 516–521 (1979).
Seidman, J. G. & Leder, P. Nature 276, 790–794 (1978).
Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. J. H. & Roe, B. A. J. molec. Biol. 143, 161–178 (1980).
Messing, J., Crea, R. & Seeburg, P. H. Nucleic Acids Res. 9, 309–321 (1981).
Larhammar, D. et al. Proc. natn. Acad. Sci. U.S.A. 79, 3687–3691 (1982).
Kratzin, H. et al. Hoppe Seyler's Z. physiol. Chem. 362, 1665–1669 (1981).
Long, E. O., Wake, C. T., Gorski, J. & Mach, B. EMBO J. 2, 389–394 (1983).
Roux-Dosseto, M. et al. Proc. natn. Acad. Sci. U.S.A. 80, 6036–6040 (1983).
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McIntyre, K., Seidman, J. Nucleotide sequence of mutant I-Aβbm12 gene is evidence for genetic exchange between mouse immune response genes. Nature 308, 551–553 (1984). https://doi.org/10.1038/308551a0
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DOI: https://doi.org/10.1038/308551a0
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