Abstract
Teratocarcinoma cells have been used as a model to study differentiation and development in vertebrates1–3. Treatment with retinoic acid (RA) and dibutyryl cyclic AMP can in some embryonal carcinoma (EC) cell lines lead to neural differentiation, as judged by neurofilament expression4,5 and by the induction of enzymes involved in cholinergic transmission6,7. Short-term culture of F9 line cells with RA and dibutyryl cyclic AMP results in a biochemically demonstrable rise in acetylcholinesterase (AChE) activity8. We now report that long-term culture of F9 cells with RA and dibutyryl cyclic AMP induces neurofilament expression, demonstrated by immunofluorescence with specific antibodies. Furthermore, if nerve growth factor (NGF)9 is also added, the developing neurone-like cells exhibit immunoreactivity to tyrosine hydroxylase, a rate-limiting enzyme of catecholamine synthesis specific for adrenergic neurones10. Immunoreactivity for Leu-enkephalin-like peptides is also induced. These results suggest that F9 cells can differentiate into cells with adrenergic characteristics.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Graham, C. F. in Concepts in Mammalian Embryogenesis (ed. Sherman, M. I.) 315–394 (MIT Press, Cambridge, 1977).
Jacob, F. Proc. R. Soc. B201, 241–270 (1978).
Martin, G. R. Science 209, 768–776 (1980).
Darmon, M., Buc-Caron, M.-H., Paulin, D. & Jacob, F. EMBO J. 1, 901–906 (1982).
Paulin, D., Jakob, H., Jacob, F., Weber, K. & Osborn, M. Differentiation 22, 90–99 (1982).
Adamson, E. D., Evans, M. J. & Magrane, G. G. Eur. J. Biochem. 79, 607–615 (1977).
Pfeiffer, S. E. et al. J. Cell Biol. 88, 57–66 (1981).
Kuff, E. L. & Fewell, J. W. Devl Biol. 77, 103–115 (1980).
Levi-Montalcini, R. & Angeletti, P. U. Physiol. Rev. 48, 534–690 (1968).
Nagatsu, T., Levitt, M. & Udenfriend, S. Biochem. biophys. Res. Commun. 14, 543–549 (1964).
Wartiovaara, J., Leivo, I., Virtanen, I., Vaheri, A. & Graham, C. F. Nature 272, 355–356 (1978).
Karnovsky, M. J. & Roots, L. J. Histochem. Cytochem. 12, 219–221 (1964).
Liesi, P., Dahl, D. & Vaheri, A. J. Cell Biol. 96, 920–924 (1983).
Levi-Montalcini, R. & Booker, B. Proc. natn. Acad. Sci. U.S.A. 42, 384–391 (1960).
Schwartz, J. & Costa, E. Brain Res. 170, 198–202 (1979).
Black, I. & Kessler, J. in Neurosection and Brain Peptides (eds Martin, J. B., Reichlin, S. & Bick, K. L.) 287–297 (Raven, New York, 1981).
Thoenen, H. & Barck, Y.-A. Physiol. Rev. 60, 1284–1323 (1980).
Walicke, P. A. & Pattersson, P. H. J. Neurosci. 1, 333–342 (1981).
Hökfelt, T. et al. Proc. R. Soc. B210, 63–77 (1980).
Eränkö, O. A. Rev. Pharmac. 7, 203–222 (1967).
Hökfelt, T., Elfvin, L.-G., Schulzberg, M., Goldstein, M. & Nilsson, G. Brain Res. 132, 29–41 (1977).
Kessler, J., Adler, J., Bohn, M. & Black, I. Science 214, 335–336 (1981).
Dahl, D. & Bignami, A. J. comp. Neurol. 76, 645–658 (1977).
Dahl, D. Biochim. biophys. Acta 668, 299–306 (1981).
Berod, A., Hartman, B. K., Keller, A., Joh, T. H. & Pujol, J. F. Brain Res. 240, 235–243 (1982).
Lamouroux, A. Proc. natn. Acad. Sci. U.S.A. 79, 3881–3885 (1981).
Wharton, J. et al. Invest. Cell Path. 2, 3–10 (1979).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Liesi, P., Rechardt, L. & Wartiovaara, J. Nerve growth factor induces adrenergic neuronal differentiation in F9 teratocarcinoma cells. Nature 306, 265–267 (1983). https://doi.org/10.1038/306265a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/306265a0
This article is cited by
-
A protein kinase A–dependent molecular switch in synapsins regulates neurite outgrowth
Nature Neuroscience (2002)
-
Immortalization of committed precursor cells from mouse teratocarcinoma using an adenovirus-SV40 recombinant plasmid
Methods in Cell Science (1995)
-
Regulated expression of an introduced MHC H–2Kbm1 gene in murine embryonal carcinoma cells
Nature (1984)
-
Differentiation of F9 teratocarcinoma stem cells after transfer of c-fos proto-oncogenes
Nature (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.