Abstract
Intermediate-sized filaments (IF) of diameter 7–11 nm occur in the cytoplasm of most cells of vertebrates and their constituent proteins are abundant in most cell types. Expression of IF proteins depends on the route of cell differentiation and five major subclasses of IF proteins have been distinguished1–5: of these, cytokeratins are typical of epithelial cells whereas vimentin occurs in mesenchymally derived cells and some other non-epithelial cells. When epithelial cells are grown in culture this restriction of IF expression is often lost and they begin to synthesize vimentin in addition to cytokeratin5–9, although examples of maintenance of the cell-type-specific expression of only cytokeratin have also been reported8,10. No IFs have been detected in mammalian germ cells or in pre-morula stages of mouse embryogenesis, and the first IF proteins identified in murine blastocysts are cytokeratins of trophectodermal cells11,12. We report here that a dedifferentiated13 rat hepatoma cell clone, which has become resistant to the action of the glucocorticoid hormone analogue dexamethasone and has lost various liver-specific functions, also stops all synthesis of IF proteins, without obvious consequences for growth and proliferation. The existence of such cells devoid of IF supports the notion that such filaments are not involved in basic cellular functions necessary for growth and proliferation but are related to special functions of the differentiated cell.
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References
Lazarides, E. Nature 283, 249–256 (1980).
Anderton, B. H. J. Muscle Res. Cell Motil. 2, 141–166 (1981).
Franke, W. W. et al. Cold Spring Harb. Symp. quant. Biol. 46, 431–453 (1982).
Osborn, M. et al. Cold Spring Harb. Symp. quant. Biol. 46, 413–429 (1982).
Franke, W. W., Schmid, E., Osborn, M. & Weber, K. Proc. natn. Acad. Sci. U.S.A. 75, 5034–5038 (1978).
Franke, W. W., Schmid, E., Weber, K. & Osborn, M. Expl. Cell Res. 118, 95–109 (1979).
Franke, W. W. et al. Differentiation 14, 35–50 (1979).
Franke, W. W. et al. Expl. Cell Res. 134, 345–365 (1981).
Virtanen, I. et al. J. Cell Sci. 50, 45–63 (1981).
Schmid, E. et al. J. Cell. Biol. 96, 37–50 (1983).
Jackson, B. W. et al. Differentiation 17, 161–179 (1980).
Paulin, D., Babinet, C., Weber, K. & Osborn, M. Expl. Cell Res. 130, 297–304 (1980).
Hay, E. D. in Cell Biology of Extracellular Matrix (ed. Hay, E. D.) 379–409 (Plenum, New York, 1981).
Deschatrette, J. & Weiss, M. C. Biochimie 56, 1603–1611 (1974).
Venetianer, A., Pinter, Zs & Gal, A. Cytogenet. Cell Genet. 28, 280–283 (1980).
Venetianer, A. & Bösze, Zs. Somat. Cell Genet. 9, 85–93 (1983).
Venetianer, A. & Bösze, Zs. Differentiation (in the press).
Franke, W. W., Denk, H., Kalt, R. & Schmid, E. Expl. Cell Res. 131, 299–318 (1981).
Deschatrette, J., Moore, E. E., Dubois, M. & Weiss, M. C. Cell 19, 1043–1051 (1980).
Franke, W. W. et al. Biol. Cell. 34, 99–110 (1979).
Franke, W. W. et al. Proc. natn. Acad. Sci. U.S.A. 80, 543–547 (1983).
Mueller, H. & Franke, W. W. J. molec. Biol. 163, 647–671 (1983).
Hubbard, A. L. & Ma, A. J. Cell Biol. 96, 230–239 (1983).
Moll, R. et al. Cell 31, 11–24 (1982).
Franke, W. W. et al. J. molec. Biol. 153, 933–959 (1981).
Switzer, R. C., Merril, C. R. & Shifrin, S. Analyt. Biochem. 98, 231–237 (1979).
Kreis, T. E. et al. Cell 32, 1125–1137 (1983).
Magin, T., Jorcano, J. & Franke, W. W. EMBO J. 2, 1387–1392 (1983).
Renner, W. et al. J. molec. Biol. 149, 285–306 (1981).
Venetianer, A., Gal, A. & Bösze, Zs. Acta biol. hung. 32, 175–187 (1981).
Lehto, V.-P., Virtanen, I. & Kurki, P. Nature 272, 175–177 (1978).
Gawlitta, W., Osborn, M. & Weber, K. Eur. J. Cell Biol. 26, 83–90 (1981).
Klymkowsky, M. W. Nature 291, 249–251 (1981).
Lane, E. B. & Klymkowsky, M. W. Cold Spring Harb. Symp. quant. Biol. 46, 387–402 (1982).
Klymkowsky, M. W., Miller, R. H. & Lane, E. B. J. Cell Biol. 96, 494–509 (1983).
Thompson, E. B. et al. in Gene Regulation by Steroid Hormone (eds Roy, A. K. & Clark, J. N.) 126–152 (Springer, Berlin, 1980).
Deschatrette, J. Cell 22, 501–511 (1980).
Franke et al. Expl. Cell Res. 123, 25–46 (1979).
Franke et al. J. Cell Biol. 84, 633–654 (1980).
Schmidt, W. N. et al. Proc. natn. Acad. Sci. U.S.A. 79, 3138–3142 (1982).
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Venetianer, A., Schiller, D., Magin, T. et al. Cessation of cytokeratin expression in a rat hepatoma cell line lacking differentiated functions. Nature 305, 730–733 (1983). https://doi.org/10.1038/305730a0
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DOI: https://doi.org/10.1038/305730a0
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