Skip to main content

Thank you for visiting nature.com. You are using a browser version with limited support for CSS. To obtain the best experience, we recommend you use a more up to date browser (or turn off compatibility mode in Internet Explorer). In the meantime, to ensure continued support, we are displaying the site without styles and JavaScript.

  • Letter
  • Published:

Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon

Nature volume 305, pages 726–729 (1983)Cite this article

Abstract

T-lymphocyte-mediated responses to the cellular components of blood vessels are important in rejection of allografts1–3. The induction of cytolytic T lymphocytes (CTLs) depends on recognition of foreign class II major histocompatibility complex antigens (human HLA-DR, DC/DS, SB and others, collectively referred to as Ia) on the target cells whereas killing by CTLs usually depends on recognition of foreign class I antigens (HLA-A,B)4, although some alloreactive CTLs recognize foreign Ia instead of HLA-A, B (refs 5–8). The expression of Ia antigens has traditionally been regarded as restricted to immunological cell types, and the presence of class II antigen-bearing ‘passenger’ leukocytes in rodent organ grafts appears necessary for graft rejection9–11. Recently, Ia antigens have been observed by immunofluorescence microscopy on human renal and dermal capillary endothelium12–15. We have previously shown that human umbilical vein endothelial (HUVE) cells in standard culture conditions do not bear Ia antigens, but may be induced to do so by products of lectin- or alloantigen-activated T lymphocytes16,17. Furthermore, we found that recombinant immune interferon (IFN-γ), free of other lymphokines, is a potent inducer of Ia expression in HUVE cells17. Here we report that IFN-γ also induces Ia expression on human foreskin capillary endothelial (HFCE) cells, HUVE cells transformed by Simian virus 40 viral DNA (SV-HUVE cells) and human dermal fibroblast (HDF) cells in culture. Further, we present evidence that Ia present on HUVE cells and HDF cells can be functionally recognized by human T cells, resulting in a two-way interaction between T cells and mesenchymal cells that may be important in allograft rejection.

This is a preview of subscription content, access via your institution

Access options

Buy this article

  • Purchase on Springer Link
  • Instant access to full article PDF
Buy now

Prices may be subject to local taxes which are calculated during checkout

Similar content being viewed by others

References

  1. Busch, G. J. et al. Lab. Invest. 35, 272–282 (1976).

    CAS  PubMed  Google Scholar 

  2. Busch, G. J., Garovoy, M. R. & Tilney, N. L. Transplant Proc. 11, 100–103 (1979).

    CAS  PubMed  Google Scholar 

  3. Dvorak, H. F. et al. J. exp. Med. 150, 322–337 (1979).

    Article  CAS  Google Scholar 

  4. Bach, F. H., Bach, M. L. & Sondel, P. M. Nature 259, 273–281 (1976).

    Article  ADS  CAS  Google Scholar 

  5. Feighery, C. & Stastny, P. J. exp. Med. 149, 485–494 (1979).

    Article  CAS  Google Scholar 

  6. Albrechtsen, D., Arnesen, E. & Thorsby, E. Transplantation 27, 338–341 (1979).

    Article  CAS  Google Scholar 

  7. Krensky, A. M., Reiss, C. S., Mier, J. W., Strominger, J. L. & Burakoff, S. J. Proc. natn. Acad. Sci. U.S.A. 79, 2365–2369 (1982).

    Article  ADS  CAS  Google Scholar 

  8. Meuer, S. C., Schlossman, S. F. & Reinherz, E. L. Proc. natn. Acad. Sci. U.S.A. 79, 4395–4399 (1982).

    Article  ADS  CAS  Google Scholar 

  9. Snell, G. A. Rev. Microbiol. 2, 439–457 (1957).

    Article  Google Scholar 

  10. Faustman, D., Hauptfeld, V., Lacy, P. & Davie, J. Proc. natn. Acad. Sci. U.S.A. 78, 5156–5159 (1981).

    Article  ADS  CAS  Google Scholar 

  11. Lechler, R. I. & Batchelor, J. R. J. exp. Med. 155, 31–41 (1982).

    Article  CAS  Google Scholar 

  12. Koyama, K., Fukunishi, T., Barcos, M., Tanigaki, N. & Pressman, D. Immunology 38, 333–341 (1979).

    CAS  PubMed  PubMed Central  Google Scholar 

  13. Natali, P. G. et al. Transplantation 31, 75–78 (1981).

    Article  CAS  Google Scholar 

  14. Baldwin, W. M. III, Claas, F. H. J., Van Es, L. A. & Van Rood, J. J. Transplant Proc. 13, 103–107 (1981).

    PubMed  Google Scholar 

  15. Hart, D. N. J. et al. Transplantation 31, 428–433 (1981).

    Article  CAS  Google Scholar 

  16. Pober, J. S. & Gimbrone, M. A. Jr Proc. natn. Acad. Sci. U.S.A. 79, 6641–6645 (1982).

    Article  ADS  CAS  Google Scholar 

  17. Pober, J. S. et al. J. exp. Med. 157, 1339–1354 (1983).

    Article  CAS  Google Scholar 

  18. Gimbrone, M. A. Jr Prog. Hemostasis Thromb. 3, 1–28 (1976).

    Google Scholar 

  19. Gimbrone, M. A. Jr & Fareed, G. C. Cell 9, 685–693 (1976).

    Article  Google Scholar 

  20. Folkman, J., Haudenschild, C. C. & Zetter, B. Z. Proc. natn. Acad. Sci. U.S.A. 76, 5217–5221 (1979).

    Article  ADS  CAS  Google Scholar 

  21. Didinsky, J. B. & Rheinwald, J. G. J. cell. Physiol. 109, 171–179 (1981).

    Article  CAS  Google Scholar 

  22. Van Rood, J. J., De Vries, R.R.P. & Bradley, B. A. in The Role of the Major Histocompatibility Complex in Immunobiology (ed. Dorf, M. E.) 59–113 (Garland STPM, New York, 1981).

    Google Scholar 

  23. Weinberger, O., Germain, R. N., Springer, T. & Burakoff, S. J. J. Immun. 129, 694–697 (1982).

    CAS  PubMed  Google Scholar 

  24. Krensky, A. M., Clayberger, C., Reiss, C. S., Strominger, J. L. & Burakoff, S. J. J. Immun. 129, 2001–2003 (1982).

    CAS  PubMed  Google Scholar 

  25. Krensky, A. M., Clayberger, C., Greenstein, J., Crimmins, M. & Burakoff, S. J. J. Immun. (in the press).

  26. Wiltrout, R. H. & Frost, P. J. Immun. 124, 2254–2263 (1980).

    CAS  PubMed  Google Scholar 

  27. Basham, T. & Merigan, T. C. Nature 299, 778 (1982).

    Article  ADS  CAS  Google Scholar 

  28. Steeg, P. S., Moore, R. N., Johnson, H. M. & Oppenheim, J. J. J. exp. Med. 156, 1780–1793 (1982).

    Article  CAS  Google Scholar 

  29. Kelley, V. E., Fiers, W. & Strom, T. B. J. Immun. (submitted).

  30. Basham, T. Y. & Merigan, T. C. J. Immun. 130, 1492–1494 (1983).

    CAS  PubMed  Google Scholar 

  31. Wong, G. H. W., Clark-Lewis, I., McKimm-Breschkin, J. L. & Schrader, J. W. Proc. natn. Acad. Sci. U.S.A. 79, 6989–6993 (1982).

    Article  ADS  CAS  Google Scholar 

  32. Wallach, D., Fellows, M. & Revel, M. Nature 299, 833–836 (1982).

    Article  ADS  CAS  Google Scholar 

  33. Scher, M. G., Beller, D. I. & Unanue, E. R. J. exp. Med. 152, 1684–1698 (1980).

    Article  CAS  Google Scholar 

  34. Lampert, I. A., Suitters, A. J. & Chisholm, P. M. Nature 293, 149–150 (1981).

    Article  ADS  CAS  Google Scholar 

  35. Mason, D. W., Dallman, M. & Barclay, A. N. Nature 293, 150–151 (1981).

    Article  ADS  CAS  Google Scholar 

  36. Suitters, A. J. & Lampert, I. A. Br. J. exp. Path. 63, 207–213 (1982).

    CAS  Google Scholar 

  37. Barclay, A. N. & Mason, D. W. J. exp. Med. 156, 1665–1676 (1982).

    Article  CAS  Google Scholar 

  38. Weil, J. et al. Nature 301, 437–439 (1983).

    Article  ADS  CAS  Google Scholar 

  39. Burger, D. R., Vetto, R. M., Hamblin, A. & Dumonde, D. C. in Pathobiology of the Endothelial Cell (eds Nossel, H. L. & Vogel, H. J.) 387–407 (Academic, New York, 1982).

    Book  Google Scholar 

  40. Hirschberg, H., Braathen, L. R. & Thorsby, E. Immun. Rev. 66, 57–77 (1982).

    Article  CAS  Google Scholar 

  41. Cosimi, A. B. et al. New Engl. J. Med. 305, 308–314 (1981).

    Article  CAS  Google Scholar 

  42. Platt, J. L., LeBien, T. W. & Michael, A. F. J. exp. Med. 155, 17–30 (1982).

    Article  CAS  Google Scholar 

  43. Scahill, S. J., Devos, R., Van der Heyden, J. & Fiers, W. Proc. natn. Acad. Sci. U.S.A. (in the press).

Download references

Author information

Authors and Affiliations

  1. Department of Pathology, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts, 02115, USA

    Jordan S. Pober, Tucker Collins, Michael A. Gimbrone Jr & Ramzi S. Cotran

  2. Department of Pediatric Medicine, Children's Hospital Medical Center, Boston, Massachusetts, 02115, USA

    Jonathan D. Gitlin

  3. Department of Pediatric Oncology, Dana-Farber Cancer Institute and Harvard Medical School, Boston, Massachusetts, 02115, USA

    Carol Clayberger, Alan M. Krensky, Steven J. Burakoff & Carol S. Reiss

  4. The Laboratory of Molecular Biology, State University of Ghent, Ghent, Belgium

    Walter Fiers

Authors
  1. Jordan S. Pober
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tucker Collins
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Michael A. Gimbrone Jr
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Ramzi S. Cotran
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jonathan D. Gitlin
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Walter Fiers
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Carol Clayberger
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Alan M. Krensky
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Steven J. Burakoff
    View author publications

    You can also search for this author in PubMed Google Scholar

  10. Carol S. Reiss
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pober, J., Collins, T., Gimbrone, M. et al. Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon. Nature 305, 726–729 (1983). https://doi.org/10.1038/305726a0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1038/305726a0

This article is cited by

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.

Search

Advanced search

Quick links

Nature Briefing

Sign up for the Nature Briefing newsletter — what matters in science, free to your inbox daily.

Get the most important science stories of the day, free in your inbox. Sign up for Nature Briefing