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The glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumour virus

Nature volume 304, pages 749–752 (1983)Cite this article

Abstract

Glucocorticoids are known to induce the transcription of integrated proviral mouse mammary tumour virus (MMTV) genes in a variety of cell lines derived from mouse mammary tumours1. Chimaeric genes in which selectable markers are linked to the long terminal repeat (LTR) region of MMTV can be induced by the synthetic glucocorticoid dexamethasone after introduction into mouse fibroblasts2–5. This suggests that the regulatory elements required for hormonal induction are located within the cloned LTR fragments. The idea is supported by the observation that glucocorticoid receptors bind to certain cloned fragments of MMTV DNA in vitro6–9. Using filter binding studies and monoclonal antibodies to the glucocorticoid receptor10 we have now delimited the receptor binding region to a DNA segment of 152 base pairs (bp) that has been shown to be relevant for hormonal induction11. In nuclease protection experiments we have identified partially homologous receptor binding sequences located in this region, all of which share the hexanucleotide 5′-TGTTCT-3′.

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Authors and Affiliations

  1. Institut für Physiologische Chemie, Philipps-Universität, D-355, Marburg, FRG

    Claus Scheidereit, Sabine Geisse, Hannes M. Westphal & Miguel Beato

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  1. Claus Scheidereit
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  2. Sabine Geisse
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  3. Hannes M. Westphal
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  4. Miguel Beato
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Scheidereit, C., Geisse, S., Westphal, H. et al. The glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumour virus. Nature 304, 749–752 (1983). https://doi.org/10.1038/304749a0

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