Abstract
Oestrogen, acting in both the brain and pituitary, has a critical role in regulating the reproductive cycle in most mammals1,2. In the brain, oestrogen regulates the release of luteinizing hormone-releasing hormone (LHRH) partly through a mechanism that is blocked by inhibitors of DNA-dependent RNA synthesis3,4 or protein synthesis4. The distributions of oestrogen-concentrating neurones5 and of LHRH neurones6,7 overlap. The present study was undertaken to determine whether genomic effects of oestrogen mediated by nuclear oestradiol concentration include a direct effect on LHRH-containing neurones. During extensive studies in which the immunocytochemical method for localizing LHRH neurones was optimized and made compatible with the autoradiographic method for detecting oestrogen-concentrating neurones, doubly-labelled cells were very rarely seen. This suggests that genomic regulatory effects of oestrogen which depend on nuclear retention are not exerted directly on most LHRH neurones, but rather must be mediated by another class of neurones.
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Shivers, B., Harlan, R., Morrell, J. et al. Absence of oestradiol concentration in cell nuclei of LHRH-immunoreactive neurones. Nature 304, 345–347 (1983). https://doi.org/10.1038/304345a0
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DOI: https://doi.org/10.1038/304345a0
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