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mRNA sequences define an unusually restricted IgG response to 2-phenyloxazolone and its early diversification

Nature volume 304, pages 320–324 (1983)Cite this article

Abstract

Light and heavy chain mRNAs of 15 anti-oxazolone antibodies were sequenced directly using synthetic primers. Hybridomas obtained 7 days after primary immunization show that they are of restricted heterogeneity. The majority expressed a single sequence with minor differences concentrated in the D region and joining boundaries D–JH and Vκ–Jκ.

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Author notes

  1. Matti Kaartinen

    Present address: Department of Bacteriology and Immunology, University of Helsinki, 00290, Helsinki, 29, Finland

  2. Alex F. Markham: ICI Pharmaceuticals Division, Mereside Alderley Park, Macclesfield, Cheshire SK10 4TG, UK

Authors and Affiliations

  1. Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, UK

    Matti Kaartinen, Gillian M. Griffiths, Alex F. Markham & Cesar Milstein

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  1. Matti Kaartinen
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  2. Gillian M. Griffiths
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  3. Alex F. Markham
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  4. Cesar Milstein
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Kaartinen, M., Griffiths, G., Markham, A. et al. mRNA sequences define an unusually restricted IgG response to 2-phenyloxazolone and its early diversification. Nature 304, 320–324 (1983). https://doi.org/10.1038/304320a0

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