Abstract
The transforming gene of a human lung carcinoma-derived cell line, Hs242, has been cloned in biologically active form, and identified as c-bas/has (otherwise known as c-Ha-ras). The genetic lesion responsible for the transforming activity of the Hs242 oncogene has been localized to a point mutation in the second exon which results in the substitution of leucine for glutamine as amino acid 61 of the predicted protein. No changes were observed in the first exon, the region of c-bas/has in which a point mutation is responsible for activation of the T24 and EJ bladder carcinoma oncogenes.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Cold Spring Harb. Symp. quant. Biol. 44, pt 2 (1980).
Frankel, A. E., Gilbert, J. H., Porzig, K. J., Scolnick, E. M. & Aaronson, S. A. J. Virol. 30, 821–827 (1979).
Shibuya, M., Hanafusa, T., Hanafusa, H. & Stephenson, J. R. Proc. natn. Acad. Sci. U.S.A. 77, 6536–6540 (1980).
Anderson, P. R. et al. Cell 26, 129–134 (1981).
Shih, C., Shilo, B. Z., Goldfarb, M. P., Dannenberg, A. & Weinberg, R. A. Proc. natn. Acad. Sci. U.S.A. 76, 5714–5718 (1979).
Cooper, G. M., Okenquist, S. & Silverman, L. Nature 284, 418–421 (1980).
Krontiris, T. G. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 78, 1181–1184 (1981).
Murray, M. J. et al. Cell 25, 355–361 (1981).
Perucho, M. et al. Cell 27, 467–476 (1981).
Pulciani, S. et al. Proc. natn. Acad. Sci. U.S.A. 79, 2845–2849 (1982).
Marshall, C. J., Hall, A. & Weise, R. A. Nature 299, 171–173 (1982).
Pulciani, S. et al. Nature 300, 539–542 (1982).
Peters, R. L., Rabstein, L. S., Van Vleck, R., Kelloff, G. J. & Huebner, R. J. J. natn. Cancer Inst. 53, 1725–1729 (1974).
Harvey, J. J. Nature 204, 1104–1105 (1964).
Ellis, R. W. et al. J. Virol. 36, 408–420 (1980).
Kirsten, W. H. & Mayer, L. A. J. natn. Cancer Inst. 39, 311–335 (1967).
Tsuchida, N., Ryder, T. & Ohtsubo, E. Science 217, 937–939 (1982).
Dhar, R. et al. Science 217, 934–937 (1982).
Ellis, R. W. et al. Nature 292, 506–511 (1981).
Der, C. J., Krontiris, T. G. & Cooper, G. M. Proc. natn. Acad. Sci. U.S.A. 79, 3637–3640 (1982).
Parada, L. F., Tabin, C. J., Shih, C. & Weinberg, R. A. Nature 297, 474–478 (1982).
Santos, E., Tronick, S. R., Aaronson, S. A., Pulciani, S. & Barbacid, M. Nature 298, 343–347 (1982).
Jelinek, W. R. et al. Proc. natn. Acad. Sci. U.S.A. 77, 1398–1402 (1980).
Shih, C. & Weinberg, R. A. Cell 29, 161–169 (1982).
Goldfarb, M., Shimizu, K., Perucho, M. & Wigler, M. Nature 296, 404–409 (1982).
Chang, E. H., Furth, M. E., Scolnick, E. M. & Lowy, D. R. Nature 297, 479–483 (1982).
Tabin, C. J. et al. Nature 300, 143–149 (1982).
Reddy, E. P., Reynolds, R. K., Santos, E. & Barbacid, M. Nature 300, 149–152 (1982).
Taparowsky, E. et al. Nature 300, 762–765 (1982).
Furth, M. E., Davis, L. J., Fleurdelys, B. & Scolnick, E. M. J. Virol. 43, 294–304 (1982).
Perler, F. et al. Cell 20, 555–566 (1980).
Miyata, T. & Yasunaga, T. Proc. natn. Acad. Sci. U.S.A. 78, 450–453 (1981).
Pierce, J. H. & Aaronson, S. A. J. exp. Med. 156, 873–887 (1982).
Weissman, B. E. & Aaronson, S. A. Cell 32, 599–606 (1983).
Graham, F. L. & Van der Erb, A. J. Virology 52, 456–467 (1973).
Wigler, M. et al. Cell 11, 223–232 (1977).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Eva, A. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Yuasa, Y., Srivastava, S., Dunn, C. et al. Acquisition of transforming properties by alternative point mutations within c-bas/has human proto-oncogene. Nature 303, 775–779 (1983). https://doi.org/10.1038/303775a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/303775a0
This article is cited by
-
An Update on the Biology of RAS/RAF Mutations in Colorectal Cancer
Current Colorectal Cancer Reports (2011)
-
Efficient modification of human chromosomal alleles using recombination-proficient chicken/human microcell hybrids
Nature Genetics (1996)
-
Neoplastic transformation and characterization of human fibroblasts by treatment with60Co gamma rays and the human c-Ha-ras oncogene
In Vitro Cellular & Developmental Biology - Animal (1993)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.