Letter | Published:

A survey of human leukaemias for sequences of a human retrovirus

Naturevolume 302pages626628 (1983) | Download Citation

Subjects

Abstract

Human T-cell leukaemia-lymphoma virus (HTLV) is an exogenous human retrovirus distinct from all known animal retroviruses. HTLV is closely linked to a subtype of adult T-cell malignancies and except for isolated cases, has not been found associated with any other form of leukaemia, lymphoma or other cancers (see refs 1, 2 for review). HTLV can be transmitted to cord blood T lymphocytes in vitro and the infected cells exhibit characteristics of transformed neoplastic T cells3–5. We have recently cloned DNA sequences derived from approximately 1 kilobase (kb) of the 5′ and 3′ termini of the HTLV genome, as well as a 4–5-kb defective HTLV provirus flanked by cellular sequences6. The availability of these probes has enabled us to carry out a limited survey of different fresh or cultured cells from patients of different lymphoid and myeloid malignancies for HTLV-related DNA sequences. The results presented here show that cells from all Japanese patients with adult T-cell leukaemia and several patients with various mature T-cell malignancies from elsewhere contained one or more copies of a highly conserved HTLV genome. The infected cells are of clonal origin. Fresh cells from 1 of the 10 myeloid leukaemic patients contained exogenous DNA sequences distantly related to HTLV.

Access optionsAccess options

Rent or Buy article

Get time limited or full article access on ReadCube.

from$8.99

All prices are NET prices.

References

  1. 1

    Gallo, R. C. & Wong-Staal, F. Blood 60, 545–557 (1982).

  2. 2

    Gallo, R. C. et al. in Modem Trends in Human Leukemia V (ed. Neth, R.) (Springer, Munich, in the press).

  3. 3

    Miyoshi, I. et al. Nature 294, 770–771 (1982).

  4. 4

    Popovic, M. et al. Science 219, 856–859 (1983).

  5. 5

    Markham, P. D. et al. J. Virol. (submitted).

  6. 6

    Manzari, V. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).

  7. 7

    Yoshida, M., Miyoshi, I. & Hinuma, Y. Proc. natn. Acad. Sci. U.S.A. 79, 2031–2035 (1982).

  8. 8

    Saxon, A., Stevens, R. H. & Golde, D. W. Ann. intern. Med. 88, 323–326 (1978).

  9. 9

    Kalyanaraman, V. S. et al. Science 218, 571–573 (1982).

  10. 10

    Gallo, R. C. et al. Cancer Res. (submitted).

  11. 11

    Neel, B. G., Hayward, W. S., Robinson, H. L., Fang, J. & Astrin, S. M. Cell 23, 323–334 (1981).

  12. 12

    Payne, G. S., Bishop, J. M. & Varmus, H. E. Nature 295, 209–213 (1982).

  13. 13

    Kettmann, R. et al. Leukemia Res. 4, 509–519 (1981).

  14. 14

    Manzari, V. et al. Proc. natn. Acad. Sci. U.S.A. 80, 11–15 (1983).

  15. 15

    Hayward, W. S., Neel, B. G. & Astrin, S. M. in Advances in Viral Oncology Vol. 1 (ed. Klein, G.) 207–233 (Raven, New York, 1982).

  16. 16

    Sarin, P. et al. Proc. natn. Acad. Sci. U.S.A. (in the press).

Download references

Author information

Affiliations

  1. Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20205, USA

    • Flossie Wong-Staal
    • , Beatrice Hahn
    • , Vittorio Manzari
    • , Sandra Colombini
    • , Genoveffa Franchini
    • , Edward P. Gelmann
    •  & Robert C. Gallo

Authors

  1. Search for Flossie Wong-Staal in:

  2. Search for Beatrice Hahn in:

  3. Search for Vittorio Manzari in:

  4. Search for Sandra Colombini in:

  5. Search for Genoveffa Franchini in:

  6. Search for Edward P. Gelmann in:

  7. Search for Robert C. Gallo in:

About this article

Publication history

Received

Accepted

Issue Date

DOI

https://doi.org/10.1038/302626a0

Further reading

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.