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A neuronal clone derived from a Rous sarcoma virus-transformed quail embryo neuroretina established culture

Naturevolume 302pages616618 (1983) | Download Citation

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Abstract

Neuroretina (NR) is an evagination of the central nervous system (CNS) which is composed of photoreceptors, glial (Müller) cells and horizontal, bipolar, amacrine and ganglion neuronal cells. We describe here the usefulness of Rous sarcoma virus (RSV) in the establishment of a neuronal clone from quail embryo neuroretina. When primary cultures of chick and quail embryo neuroretina cells are transformed by RSV, neuronal markers such as ribbon synapses, choline acetyltransferase (CAT) and glutamic acid decarboxylase (GAD) specific activity are present1. These RSV-transformed primary cultures can be established into permanent cell lines from which neuronal clones have been isolated. One of them, clone QNR/D, can generate tetrodotoxin(TTX)-inhibitable action potentials on electrical stimulation, has a high GAD activity and binds monoclonal antibodies raised against chick embryo neuroretina. The pres-ence of these neuronal markers suggests that the QNR/D clone is derived from cells of the amacrine or ganglionic lineage. This is the first time that a neuronal cell clone of defined origin has been obtained from the CNS. The neuronal markers of the QNR/D clone are expressed at both the permissive and the non-permissive temperatures for transformation.

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Author information

Affiliations

  1. CNRS ER 231 and INSERM U178, Hôpital Broussais, 96 rue Didot, 75674, Paris, Cedex 14, France

    • Bernard Pessac
    • , Arlette Girard
    • , Patricia Crisanti
    •  & Anne-Marie Lorinet
  2. Centre de Biochimie, CNRS, Université de Nice, Parc Valrose, 06034, Nice, France

    • Georges Romey
  3. Institut Curie-Biologie, Bâtiment 110, 91405, Orsay Cedex, France

    • Georges Calothy

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https://doi.org/10.1038/302616a0

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