Abstract
Among the proteins encoded by the major histocompatibility complex (MHC) are the highly polymorphic class I major transplantation antigens, HLA-A and HLA-B in man and H–2K and H–2D in mouse. Class I loci also include the less polymorphic HLA-C in man and H–2L in mouse. Class I antigens are 45,000-molecular weight (Mr) glycosylated membrane proteins associated on the cell surface with apparently nonpolymorphic β2-microglobulin1. Linked to the murine MHC are genes encoding the class I-like Qa and Tla antigens, which are closely related structurally to H–2K, D and L, as they are also 45,000-Mr cell surface glycoproteins associated with β2-microglobulin2–7. Such class I molecules have also been found on the surface of human T lymphocytes8–11 and, in one case, shown to be linked to HLA-A12. Recombinant DNA techniques have been used to map DNA fragments to the Tla region in mouse13,14. A similar genetic analysis of the HLA complex is hampered by the lack of congeneic strains and by the small number of intra-MHC recombinants in well studied families. One means of overcoming these problems involves a molecular genetic analysis of human lymphoblastoid cell line (LCL) mutants15 having γ-ray-induced physical deletions of HLA DNA to map DNA fragments in the MHC16. Here we have applied this method to provide evidence that some human class I-like DNA sequences are telomeric to the A2 locus in LCL 721.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Ploegh, H., Orr, H. T. & Strominger, J. L. Cell 24, 287–299 (1981).
Rask, L., Lindblom, J. B. & Peterson, P. A. Nature 249, 833–834 (1974).
Silver, J. & Hood, L. Nature 249, 764–765 (1974).
Michaelson, J., Flaherty, L., Vitetta, E. & Poulik, M. J. exp. Med. 145, 1066–1070 (1977).
Stanton, T. H. & Hood, L. Immunogenetics 11, 309–318 (1980).
Michaelson, J. Immunogenetics 13, 167–171 (1981).
Flaherty, L. in The Role of the Major Histocompatibility Complex in Immunobiology (ed. Dorf, M. E.) 33–58 (Garland STPM, New York, 1980).
Tada, N., Tanigaki, N. & Pressman, D. J. Immun. 120, 513–519 (1978).
Ziegler, A. & Milstein, C. Nature 279, 243–244 (1979).
Brodsky, F. M., Bodmer, W. F. & Parham, P. Eur. J. Immun. 9, 536–545 (1979).
Cotner, T., Mashimo, H., Kung, P. C., Goldstein, G. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 78, 3858–3862 (1981).
Gazit, E., Terhorst, C. & Yunis, E. J. Nature 284, 275–277 (1980).
Margulies, D. H., Evans, G. A., Flaherty, L. & Seidman, J. G. Nature 295, 168–170 (1982).
Pease, L. R., Nathenson, S. G. & Leinwand, L. A. Nature 298, 382–385 (1982).
Kavathas, P., Bach, F. H. & DeMars, R. Proc. natn. Acad. Sci. U.S.A. 77, 4251–4255 (1980).
Orr, H. T. et al. Nature 296, 454–456 (1982).
Sood, A. K., Pereira, D. & Weissman, S. M. Proc. natn. Acad. Sci. U.S.A. 78, 616–620 (1981).
Ploegh, H. L., Orr, H. T. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 77, 6081–6085 (1980).
Yokayama, K., Stockert, E., Old, L. V. & Nathenson, S. G. Proc. natn. acad. Sci. U.S.A. 78, 7078–7082 (1981).
Soloski, M. J., Uhr, J. W. & Vitetta, E. S. Nature 296, 759–761 (1982).
Shaw, J., Kavathas, P., Pollack, M. S., Charmot, D. & Mawas, C. Nature 293, 745–747 (1981).
Kavathas, P., DeMars, R., Bach, F. H. & Shaw, S. Nature 293, 747–749 (1981).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Orr, H., DeMars, R. Class I-like HLA genes map telomeric to the HLA-A2 locus in human cells. Nature 302, 534–536 (1983). https://doi.org/10.1038/302534a0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1038/302534a0
This article is cited by
-
Lack of close linkage between human coagulation factor F13A and HLA class I molecules
Immunogenetics (1989)
-
Organization of the human class I major histocompatibility complex genes
Immunologic Research (1987)
-
The major histocompatibility complex of primates
Genetica (1987)
-
Alloantisera with apparent HLA-A specificity reacting only against phytohemagglutinin-activated cells
La Ricerca in Clinica e in Laboratorio (1986)
-
HLA-JY328: Mapping studies and expression of a polymorphic HLA class I gene
Immunogenetics (1986)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.