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Class I-like HLA genes map telomeric to the HLA-A2 locus in human cells

Naturevolume 302pages534536 (1983) | Download Citation



Among the proteins encoded by the major histocompatibility complex (MHC) are the highly polymorphic class I major transplantation antigens, HLA-A and HLA-B in man and H–2K and H–2D in mouse. Class I loci also include the less polymorphic HLA-C in man and H–2L in mouse. Class I antigens are 45,000-molecular weight (Mr) glycosylated membrane proteins associated on the cell surface with apparently nonpolymorphic β2-microglobulin1. Linked to the murine MHC are genes encoding the class I-like Qa and Tla antigens, which are closely related structurally to H–2K, D and L, as they are also 45,000-Mr cell surface glycoproteins associated with β2-microglobulin2–7. Such class I molecules have also been found on the surface of human T lymphocytes8–11 and, in one case, shown to be linked to HLA-A12. Recombinant DNA techniques have been used to map DNA fragments to the Tla region in mouse13,14. A similar genetic analysis of the HLA complex is hampered by the lack of congeneic strains and by the small number of intra-MHC recombinants in well studied families. One means of overcoming these problems involves a molecular genetic analysis of human lymphoblastoid cell line (LCL) mutants15 having γ-ray-induced physical deletions of HLA DNA to map DNA fragments in the MHC16. Here we have applied this method to provide evidence that some human class I-like DNA sequences are telomeric to the A2 locus in LCL 721.

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  1. 1

    Ploegh, H., Orr, H. T. & Strominger, J. L. Cell 24, 287–299 (1981).

  2. 2

    Rask, L., Lindblom, J. B. & Peterson, P. A. Nature 249, 833–834 (1974).

  3. 3

    Silver, J. & Hood, L. Nature 249, 764–765 (1974).

  4. 4

    Michaelson, J., Flaherty, L., Vitetta, E. & Poulik, M. J. exp. Med. 145, 1066–1070 (1977).

  5. 5

    Stanton, T. H. & Hood, L. Immunogenetics 11, 309–318 (1980).

  6. 6

    Michaelson, J. Immunogenetics 13, 167–171 (1981).

  7. 7

    Flaherty, L. in The Role of the Major Histocompatibility Complex in Immunobiology (ed. Dorf, M. E.) 33–58 (Garland STPM, New York, 1980).

  8. 8

    Tada, N., Tanigaki, N. & Pressman, D. J. Immun. 120, 513–519 (1978).

  9. 9

    Ziegler, A. & Milstein, C. Nature 279, 243–244 (1979).

  10. 10

    Brodsky, F. M., Bodmer, W. F. & Parham, P. Eur. J. Immun. 9, 536–545 (1979).

  11. 11

    Cotner, T., Mashimo, H., Kung, P. C., Goldstein, G. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 78, 3858–3862 (1981).

  12. 12

    Gazit, E., Terhorst, C. & Yunis, E. J. Nature 284, 275–277 (1980).

  13. 13

    Margulies, D. H., Evans, G. A., Flaherty, L. & Seidman, J. G. Nature 295, 168–170 (1982).

  14. 14

    Pease, L. R., Nathenson, S. G. & Leinwand, L. A. Nature 298, 382–385 (1982).

  15. 15

    Kavathas, P., Bach, F. H. & DeMars, R. Proc. natn. Acad. Sci. U.S.A. 77, 4251–4255 (1980).

  16. 16

    Orr, H. T. et al. Nature 296, 454–456 (1982).

  17. 17

    Sood, A. K., Pereira, D. & Weissman, S. M. Proc. natn. Acad. Sci. U.S.A. 78, 616–620 (1981).

  18. 18

    Ploegh, H. L., Orr, H. T. & Strominger, J. L. Proc. natn. Acad. Sci. U.S.A. 77, 6081–6085 (1980).

  19. 19

    Yokayama, K., Stockert, E., Old, L. V. & Nathenson, S. G. Proc. natn. acad. Sci. U.S.A. 78, 7078–7082 (1981).

  20. 20

    Soloski, M. J., Uhr, J. W. & Vitetta, E. S. Nature 296, 759–761 (1982).

  21. 21

    Shaw, J., Kavathas, P., Pollack, M. S., Charmot, D. & Mawas, C. Nature 293, 745–747 (1981).

  22. 22

    Kavathas, P., DeMars, R., Bach, F. H. & Shaw, S. Nature 293, 747–749 (1981).

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  1. Department of Laboratory Medicine/Pathology and Department of Microbiology, Immunobiology Research Center, University of Minnesota, 55455, USA

    • Harry T. Orr
  2. Laboratory of Genetics and Department of Human Oncology, University of Wisconsin, Madison, Wisconsin, 53706, USA

    • Robert DeMars


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