Letter | Published:

Evidence that the insulin secretagogue, β-cell-tropin, is ACTH22–39

Nature volume 301, pages 255258 (20 January 1983) | Download Citation

Subjects

Abstract

The pituitary neurointermediate lobe of genetically obese (ob/ob) mice contains a hormone which stimulates insulin release1–3 and which cross-reacts with a -COOH-terminal ACTH antiserum, suggesting that it is related to corticotropin-like intermediate lobe peptide (CLIP), the 18–39 fragment of ACTH4–6. The hormone, which we have called β-cell-tropin7, has been shown to be present in the plasma of the ob/ob mouse8 and to potentiate glucose induced insulin secretion9. We have now shown that ACTH22–39 prepared by tryptic digestion of human synthetic CLIP behaves similarly on Biogel chromatography and on reverse-phase HPLC to the naturally occurring β-cell-tropin. Furthermore, β-cell-tropin and ACTH22–39 have indistinguishable antigenic and insulin releasing properties.

References

  1. 1.

    , & J. Endocr. 65, 109–116 (1975).

  2. 2.

    , & FEBS Lett. 55, 72–74 (1975).

  3. 3.

    , , & J. Physiol., Lond. 275, 55P (1977).

  4. 4.

    et al. Nature new Biol. 244, 65–67 (1973).

  5. 5.

    , & J. Endocr. 81, 271–279 (1979).

  6. 6.

    , & J. Endocr. 73, 28P–29P (1976).

  7. 7.

    , & FEBS Lett. 117, 303–307 (1980).

  8. 8.

    , & J. Endocr. 94, 125–130 (1982).

  9. 9.

    , , , & Biochem. Soc. Trans. 9, 522–524 (1981).

  10. 10.

    & J. Endocr. 92, 15–21 (1982).

  11. 11.

    , , & JCS chem. Commun. 325–327 (1981).

  12. 12.

    , , , & Biochem. biophys. Res. Commun. 101, 623–631 (1981).

  13. 13.

    et al. Pure appl. Chem. 54, 267–279 (1982).

  14. 14.

    & Biochem. J. 135, 833–837 (1973).

  15. 15.

    , & Helv. chim. Acta 55, 1243–1248 (1972).

  16. 16.

    , & Biochemistry 20, 4538–4546 (1981).

  17. 17.

    & in Animal Models of Obesity (ed. Festing, M. J. W.) 177–190 (Macmillan, London, 1979).

  18. 18.

    , , & J. Neurochem. 34, 574–582 (1980).

Download references

Author information

Affiliations

  1. Department of Biochemistry, Imperial College of Science and Technology, London SW7 2AZ, UK

    • Anne Beloff-Chain
    • , John Morton
    • , Simon Dunmore
    • , Graham W. Taylor
    •  & Howard R. Morris

Authors

  1. Search for Anne Beloff-Chain in:

  2. Search for John Morton in:

  3. Search for Simon Dunmore in:

  4. Search for Graham W. Taylor in:

  5. Search for Howard R. Morris in:

About this article

Publication history

Received

Accepted

Published

DOI

https://doi.org/10.1038/301255a0

Comments

By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.