Arrangement of human immunoglobulin heavy chain constant region genes implies evolutionary duplication of a segment containing γ, ε and α genes

Abstract

Cosmid clones containing the human γ, ε and α heavy chain constant region genes and an ε pseudogene have been isolated. All these genes have a switch sequence detectable by hybridization. We have studied overlapping cosmids covering two separate regions of the genome, and the gene order in each of these regions was found to be γ–γ–ε–α. This implies an evolutionary duplication in this multigene family involving γ, ε and α genes.

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References

  1. 1

    Efstratiadis, A. et al. Cell 21, 653–668 (1980).

  2. 2

    Lauer, J., Shen, C.-K. J. & Maniatis, T. Cell 20, 119–130 (1980).

  3. 3

    Goeddel, D. V. et al. Nature 290, 20–26 (1981).

  4. 4

    Moore, D. D., Conkling, M. A. & Goodman, H. M. Cell 29, 285–286 (1982).

  5. 5

    Hood, L., Steinmetz, M. & Goodenow, R. Cell 28, 685–687 (1982).

  6. 6

    Davis, M. M. et al. Nature 283, 733–739 (1980).

  7. 7

    Early, P., Huang, H., Davis, M., Calame, K. & Hood, L. Cell 19, 981–992 (1980).

  8. 8

    Sakano, H., Maki, R., Kurosawa, Y., Roeder, W. & Tonegawa, S. Nature 286, 676–683 (1980).

  9. 9

    Honjo, T. & Kataoka, T. Proc. natn. Acad. Sci. U.S.A. 75, 2140–2144 (1978).

  10. 10

    Rabbitts, T. H., Forster, A., Dunnick, W. & Bentley, D. L. Nature 283, 351–356 (1980).

  11. 11

    Cory, S., Jackson, J. & Adams, J. M. Nature 285, 450–456 (1980).

  12. 12

    Coleclough, C., Cooper, C. & Perry, R. P. Proc. natn. Acad. Sci. U.S.A. 77, 1422–1426 (1980).

  13. 13

    Rabbitts, T. H., Hamlyn, P. H., Matthyssens, G. & Roe, B. A. Can. J. Biochem. 58, 176–187 (1980).

  14. 14

    Dunnick, W., Rabbitts, T. H. & Milstein, C. Nature 286, 669–675 (1980).

  15. 15

    Obata, M. et al. Proc. natn. Acad. Sci. U.S.A. 78, 2437–2441 (1981).

  16. 16

    Davis, M. M., Kim, S. K. & Hood, L. Science 209, 1360–1365 (1980).

  17. 17

    Kataoka, T., Miyata, T. & Honjo, T. Cell 23, 357–368 (1981).

  18. 18

    Shimizu, A., Takahashi, N., Yaoita, Y. & Honjo, T. Cell 28, 499–506 (1982).

  19. 19

    Rabbitts, T. H., Forster, A. & Milstein, C. P. Nucleic Acids Res. 9, 4509–4524 (1981).

  20. 20

    Ravetch, J. V., Kirsch, I. R. & Leder, P. Proc. natn. Acad. Sci. U.S.A. 77, 6734–6738 (1980).

  21. 21

    Takahashi, N., Nakai, S. & Honjo, T. Nucleic Acids Res. 8, 5983–5991 (1980).

  22. 22

    Takahashi, N. et al. Cell 29, 671–679 (1982).

  23. 23

    Krawinkel, U. & Rabbits, T. H. EMBO J. 1, 403–407 (1982).

  24. 24

    Max, E. E., Battey, J., Ney, R., Kirsch, I. R. & Leder, P. Cell 29, 691–699 (1982).

  25. 25

    Flanagan, J. G. & Rabbitts, T. H. EMBO J. 1, 655–660 (1982).

  26. 26

    Nishida, Y., Miki, T., Hisajima, H. & Honjo, T. Proc. natn. Acad. Sci. U.S.A. 79, 3833–3837 (1982).

  27. 27

    Ravetch, J. V., Siebenlist, U., Korsmeyer, S., Waldmann, T. & Leder, P. Cell 27, 583–591 (1981).

  28. 28

    Ellison, J. & Hood, L. Proc. natn. Acad. Sci. U.S.A. 79, 1984–1988 (1982).

  29. 29

    Early, P. W., Davis, M. M., Kaback, D. B., Davidson, N. & Hood, L. Proc. natn. Acad. Sci. U.S.A. 76, 857–861 (1979).

  30. 30

    Messing, J. & Vieira, J. Gene 19 (in the press).

  31. 31

    Sanger, F., Coulson, A. R., Barrell, B. G., Smith, A. J. H. & Roe, B. A. J. molec. Biol. 143, 161–178 (1980).

  32. 32

    Tsuzukida, Y., Wang, C.-C. & Putnam, F. W. Proc. natn. Acad. Sci. U.S.A. 76, 1104–1108 (1979).

  33. 33

    Marcu, K. B., Lang, R. B., Stanton, L. W. & Harris, L. J. Nature 298, 87–89 (1982).

  34. 34

    Stanton, L. W. & Marcu, K. B. Nucleic Acids Res. (in the press).

  35. 35

    Lefranc, M.-P., Lefranc, G. & Rabbits, T. H. Nature 300, 760–762 (1982).

  36. 36

    Milstein, C. & Munro, A. J. in Defence and Recognition (ed. Porter, R. R.) 199–228 (Butterworths, London, 1973).

  37. 37

    Wahl, G. M., Stern, M. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 76, 3683–3687 (1979).

  38. 38

    Grosveld, F. G., Dahl, H.-H. M., deBoer, E. & Flavell, R. Gene 13, 227–237 (1981).

  39. 39

    Southern, E. M. J. molec. Biol. 98, 503–517 (1975).

  40. 40

    Jeffreys, A. J. & Flavell, R. A. Cell 12, 429–439 (1977).

  41. 41

    Laskey, R. A. & Mills, A. D. FEBS Lett. 82, 314–316 (1977).

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Flanagan, J., Rabbitts, T. Arrangement of human immunoglobulin heavy chain constant region genes implies evolutionary duplication of a segment containing γ, ε and α genes. Nature 300, 709–713 (1982). https://doi.org/10.1038/300709a0

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