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Relaxation of muscle fibres by photolysis of caged ATP

Nature volume 300, pages 701–705 (1982)Cite this article

Abstract

A novel method has been developed for studying the reaction kinetics of the force-generating mechanism in muscle. Inert photolabile precursors of ATP or ADP are incorporated into muscle fibres having their surface membrane barrier removed. The nucleotide is then rapidly liberated by laser pulse photolysis. This circumvents the limitation in time resolution set by diffusion of nucleotide from the medium bathing the fibre. This laser photolysis method may be applicable to studies of the dynamic properties of many biological systems.

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Author information

Authors and Affiliations

  1. Department of Physiology, School of Medicine, University of Pennsylvania, Philadelphia, 19104, USA

    Y. E. Goldman & M. G. Hibberd

  2. Department of Biochemistry and Biophysics, School of Medicine, University of Pennsylvania, Philadelphia, 19104, USA

    D. R. Trentham

  3. Department of Physics and Atmospheric Sciences, Drexel University, Philadelphia, Pennsylvania, 19104, USA

    J. A. McCray

Authors
  1. Y. E. Goldman
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  2. M. G. Hibberd
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  3. J. A. McCray
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  4. D. R. Trentham
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Goldman, Y., Hibberd, M., McCray, J. et al. Relaxation of muscle fibres by photolysis of caged ATP. Nature 300, 701–705 (1982). https://doi.org/10.1038/300701a0

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