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A fragment of the SV40 large T-antigen gene transforms

Nature volume 299, pages 59–61 (1982)Cite this article

Abstract

SV40-transformed cells retain and express all the sequences encoding large T antigen1; this protein, in its entirety, appears to be essential for transformation1,2. We have isolated3 a segment of integrated viral DNA (SV3T3-20-G) which encodes only the N-terminal half of T antigen hut is capable of transforming rat fibroblasts. SV3T3-20-G-transformed cells synthesize a truncated T antigen of apparent molecular weight (MW) 48,000. The sequence of the virus–host junction shows that this protein is partially encoded by the flanking mouse DNA and suggests that the SV40 DNA has integrated next to a mouse gene. The mouse DNA segment of SV3T3-20-G is not required for transformation although transcription of these flanking sequences may be activated by the insertion of viral DNA.

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Author notes

  1. Christine E. Clayton

    Present address: Department of Biochemistry, Stanford University Medical Center, Stanford, California, 94305, USA

  2. Michael Lovett: Department of Pediatrics, University of California Medical School, San Francisco, California 94143, USA.

Authors and Affiliations

  1. Cancer Research Campaign, Eukaryotic Molecular Genetics Research Group, Department of Biochemistry, Imperial College of Science and Technology, London, SW7 2AZ, UK

    Christine E. Clayton, David Murphy, Michael Lovett & Peter W. J. Rigby

Authors
  1. Christine E. Clayton
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  2. David Murphy
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  3. Michael Lovett
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  4. Peter W. J. Rigby
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Clayton, C., Murphy, D., Lovett, M. et al. A fragment of the SV40 large T-antigen gene transforms. Nature 299, 59–61 (1982). https://doi.org/10.1038/299059a0

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