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Chemical evidence for the capsomeric structure of phage Qβ

Abstract

Novel capsomeric complexes, pentamers and hexamers were detected as chemical entities in phage Qβ. Both were composed of identical protein subunits and stabilized by intermodular disulphide bonds. Their numbers per particle were about 12 for pentamers and about 20 for hexamers—consistent with theoretical expectation from the quasi-equivalent packing of 180 identical subunits in a coat protein shell.

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References

  1. Loeb, T. & Zinder, N. D. Proc. natn. Acad. Sci. U.S.A. 47, 282–289 (1961).

    Article  ADS  CAS  Google Scholar 

  2. Watanabe, I., Nishihara, T., Kaneko, H., Sakurai, T. & Osawa, S. Proc. Jap. Acad. 43, 210–213 (1967).

    Article  CAS  Google Scholar 

  3. Miyake, T. et al. Proc. natn. Acad. Sci. U.S.A. 68, 2022–2024 (1971).

    Article  ADS  CAS  Google Scholar 

  4. Caspar, D. L. D. & Klug, A. Cold Spring Harb. Symp. quant. Biol. 27, 1–24 (1962).

    Article  CAS  Google Scholar 

  5. Hohn, T. & Hohn, B. Adv. Virus Res. 16, 43–98 (1970).

    Article  CAS  Google Scholar 

  6. Crowther, R. A., Amos, L. A. & Finch, J. T. J. molec. Biol. 98, 631–635 (1975).

    Article  CAS  Google Scholar 

  7. Horiuchi, K., Webster, R. E. & Matsuhashi, S. Virology 45, 429–439 (1971).

    Article  CAS  Google Scholar 

  8. Matthews, K. C. & Cole, R. D. J. molec. Biol. 65, 1–15 (1972).

    Article  CAS  Google Scholar 

  9. Weber, K. & Konigsberg, W. in RNA Phages (ed. Zinder, N. D.) 51–84 (Cold Spring Harbor Laboratory, New York, 1975).

    Google Scholar 

  10. Weiner, A. M., Platt, T. & Weber, K. J. biol. Chem. 247, 3242–3251 (1972).

    CAS  PubMed  Google Scholar 

  11. Konigsberg, W., Maita, T., Katze, J. & Weber, K. Nature 227, 271–273 (1970).

    Article  ADS  CAS  Google Scholar 

  12. Moore, C. H., Farron, F., Bohnert, C. & Weissmann, C. Nature new Biol. 234, 204–206 (1971).

    Article  CAS  Google Scholar 

  13. Weiner, A. M. & Weber, K. Nature new Biol. 234, 206–209 (1971).

    Article  CAS  Google Scholar 

  14. Strauss, E. G. & Kaesberg, P. Virology 42, 437–452 (1970).

    Article  CAS  Google Scholar 

  15. Furuse, K. et al. Virology 97, 328–341 (1979).

    Article  CAS  Google Scholar 

  16. Overby, L. R., Barlow, G. H., Doi, R. H., Jacob, M. & Spiegelman, S. J. Bact. 91, 442–448 (1966).

    CAS  PubMed  Google Scholar 

  17. Boedtker, H. & Gesteland, R. F. in RNA Phages (ed. Zinder, N. D.) 1–28 (Cold Spring Harbor Laboratory, New York, 1975).

    Google Scholar 

  18. Glazer, A. N. in The Proteins Vol. 2 (eds Neurath, H. & Hill, R. L.) 1–103 (Academic, New York, 1976).

    Book  Google Scholar 

  19. Glazer, A. N., Delange, R. J. & Sigman, D. S. in Chemical Modification of Proteins, 113–114 (Elsevier, New York, 1975).

    Google Scholar 

  20. Hofstetter, H., Monstein, H.-J. & Weissmann, C. Biochim. biophys. Acta 374, 238–251 (1974).

    Article  CAS  Google Scholar 

  21. Watanabe, I., Miyake, T., Sakurai, T., Shiba, T. & Ohno, T. Proc. Jap. Acad. 43, 204–209 (1967).

    Article  CAS  Google Scholar 

  22. Gorovsky, M. A., Carlson, K. & Rosenbaum, J. L. Analyt. Biochem. 35, 359–370 (1970).

    Article  CAS  Google Scholar 

  23. Funakoshi, H. & Iso, K. J. Biochem. 70, 201–204 (1971).

    Article  CAS  Google Scholar 

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Takamatsu, H., Iso, K. Chemical evidence for the capsomeric structure of phage Qβ. Nature 298, 819–824 (1982). https://doi.org/10.1038/298819a0

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