Abstract
The centriolar region is a complex structural entity comprising not only centrioles, but also an abundance of associated electron-dense material identified at present only at the ultrastructural level1,2. It is known to contain initiation sites necessary for the assembly of both cytoplasmic and spindle microtubules. Basal bodies, which are structurally related to centrioles, contain the template and serve as initiation sites for the assembly of other microtubule-containing structures, such as the axonemes of cilia and flagella. Immunoglobulins in the sera of some apparently normal rabbits have the ability to bind specifically to centrioles and basal bodies, and have allowed the distribution of these structures to be localized at the light microscope level by immunofluorescence3–5. The identity of the molecule(s) detected by these sera in the centriolar region is unknown, although it is not tubulin, the major identified protein of centrioles4. We describe here the identification of a protein recognized by the immunoglobulins in some normal rabbit sera, of approximate molecular weight (MW) 50,000 in both basal bodies isolated from Tetrahymena pyriformis and in extracts of chicken tracheal epithelial cells.
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Turksen, K., Aubin, J. & Kalnins, V. Identification of a centriole-associated protein by antibodies present in normal rabbit sera. Nature 298, 763–765 (1982). https://doi.org/10.1038/298763a0
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DOI: https://doi.org/10.1038/298763a0
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