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Immunotherapy in nude mice of human hepatoma using monoclonal antibodies against hepatitis B virus

Nature volume 298, pages 567–569 (1982)Cite this article

Abstract

The human hepatoma cell line PLC/PRF/5, which contains integrated hepatitis B virus DNA, synthesises and secretes hepatitis B virus surface antigen (HBsAg)1,2. When injected into BALB/c nude mice, these cells produce well-vascularized, encapsulated tumours in almost 100% of animals1. HBsAg has been demonstrated on the surface and in the cytoplasm of PLC/PRF/5 cells using immunofluorescence techniques1,3–5. Recently, we reported that monoclonal antibodies to HBsAg (IgG1, IgG2a and IgM isotypes) bind to HBsAg-associated viral determinants of PLC/PRF/5 cells in culture and immunoprecipitate HBsAg secreted into the growth medium6. Also IgG2a and IgM antibodies to HBsAg (anti-HBs) produce specific lysis of PLC/PRF/5 cells in culture in the presence of complement, but have no lytic effect on human hepatoma cells which do not express HBsAg6. In the present study, we demonstrate that IgM and IgG2a, but not IgG1, monoclonal anti-HBs specifically prevent or suppress tumour formation in a substantial number of athymic nude mice injected with PLC/PRF/5 cells.

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Author notes

  1. Vincent R. Zurawski: Centocor, Malvern, Pennsylvania 19355, USA

  2. Kurt J. Isselbacher and Jack R. Wands: Gastrointestinal Unit, Massachusetts General Hospital, Boston, Massachusetts 02114, USA

Authors and Affiliations

  1. Liver Research Center, Department of Medicine and Cell Biology, Albert Einstein College of Medicine, Bronx, New York, 10461, USA

    Daniel Shouval, David A. Shafritz, Vincent R. Zurawski Jr, Kurt J. Isselbacher & Jack R. Wands

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  1. Daniel Shouval
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  2. David A. Shafritz
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  3. Vincent R. Zurawski Jr
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  4. Kurt J. Isselbacher
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  5. Jack R. Wands
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Shouval, D., Shafritz, D., Zurawski, V. et al. Immunotherapy in nude mice of human hepatoma using monoclonal antibodies against hepatitis B virus. Nature 298, 567–569 (1982). https://doi.org/10.1038/298567a0

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