Abstract
Cytolytic T lymphocyte (CTL) proliferation is dependent on T-cell growth factor (TCGF). However, fusion of mouse CTLs with T lymphoma cells yields two types of hybrids, cytolytic ones in which proliferation remains dependent on TCGF and others which express neither this dependence nor cytolytic function1. Clones of the first type can generate variants of the second type. TCGF-independent hybrids2 remain non-cytolytic when cultured in medium supplemented with supernatant of concanavalin A (Con A)-stimulated rat spleen cells (CS) which is our standard source of TCGF. Here we report that in TCGF-independent hybrids between a murine CTL line and a rat T lymphoma, high cytolytic activity can be induced by the addition of CS to the medium. The induction is reversible and seems to depend on a factor in CS which is different from TCGF and from immune interferon.
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Conzelmann, A., Corthésy, P., Cianfriglia, M. et al. Hybrids between rat lymphoma and mouse T cells with inducible cytolytic activity. Nature 298, 170–172 (1982). https://doi.org/10.1038/298170a0
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DOI: https://doi.org/10.1038/298170a0
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