Abstract
The principal secretory protein in rat prostatic fluid is a steroid binding protein1–6 whose expression is stimulated by androgenic steroids7,8. This prostatic steroid binding protein is oligomeric, consisting of two subunits, one containing the polypeptides C1 and C3 and the other containing C2 and C39. We have isolated cDNA clones specific for each of the mRNAs which code for prostatic binding protein and note here the considerable DNA sequence homology between C1 and C2. Therefore, it is likely that duplication of an ancestral gene occurred and the subsequent divergence results in the expression of two related polypeptides that form part of a single protein. Furthermore, in view of the structure of the protein it is possible that the divergence of one of the genes has resulted in the capacity of this prostatic protein to bind steroids.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Heyns, W., Peeters, B. & Mous, J. Biochem. biophys. Res. Commun. 77, 1492–1499 (1977).
Heyns, W. & De Moor, P. Eur. J. Biochem. 78, 221–230 (1977).
Parker, M. G., Scrace, G. T. & Mainwaring, W. I. P. Biochem. J. 170, 115–121 (1978).
Forsgren, B. et al. Proc. natn. Acad. Sci. U.S.A. 76, 3149–3153 (1979).
Lea, O. A., Petrusz, P. & French, F. S. J. biol. Chem. 254, 6196–6202 (1979).
Chen, C., Hirpakha, R. A. & Liao, S. J. Steroid Biochem. 11, 401–405 (1979).
Parker, M. G. & Mainwaring, W. I. P. Cell 12, 401–407 (1977).
Parker, M. G. & Scrace, G. T. Proc. natn. Acad. Sci. U.S.A. 76, 1580–1584 (1979).
Heyns, W., Peeters, B., Mous, J., Rombauts, W. & De Moor, P. Eur. J. Biochem. 89, 181–186 (1978).
Parker, M. G., White, R. & Williams, J. G. J. biol. Chem. 255, 6996–7001 (1980).
Maniatis, T., Kee, S. G., Efstratiadis, A. & Kafatos, F. C. Cell 8, 163–182 (1976).
Rougeon, F., Kourilsky, P. & Mach, B. Nucleic Acids Res. 2, 2365–2378 (1975).
Land, H., Grez, M., Hauser, H., Lindenmaier, W. & Schütz, G. Nucleic Acids Res. 9, 2251–2266 (1981).
Twigg, A. J. & Sherratt, D. Nature 283, 216–218 (1980).
Grunstein, M. & Hogness, D. S. Proc. natn. Acad. Sci. U.S.A. 72, 3961–3965 (1975).
Alwine, J. C., Kemp, D. J. & Stark, G. R. Proc. natn. Acad. Sci. U.S.A. 74, 5350–5354 (1977).
Peeters, B. L., Mous, J. M., Rombauts, W. A. & Heyns, W. J. J. biol. Chem. 255, 7017–7023 (1980).
Mansson, P. E., Silverberg, A. B., Gipson, S. H. & Harris, S. E. Molec. cell. Endocr. 19, 229–241 (1980).
Maxam, A. H. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Peeters, B., Rombauts, W., Mous, J. & Heyns, W. Eur. J. Biochem. 115, 115–121 (1981).
Peeters, B., Heyns, W., Mous, J. & Rombauts, W. Eur. J. Biochem. 123, 55–62 (1982).
Austen, B. M. FEBS Lett. 103, 308–313 (1979).
Jacq, C., Miller, J. R. & Brownlee, G. G. Cell 12, 109–120 (1977).
Hardison, R. C. et al. Cell 18, 1285–1297 (1979).
Fritsch, E. F., Lawn, R. M. & Maniatis, T. Cell 19, 959–972 (1980).
Royal, A. et al. Nature 279, 125–132 (1979).
Colbert, D. et al. Biochemistry 19, 5586–5592 (1980).
Wood, W. G. et al. Cell 15, 437–446 (1978).
Lomadico, P. et al. Cell 18, 545–558 (1979).
Wahli, W. & Dawid, I. B. Proc. natn. Acad. Sci. U.S.A. 77, 1437–1441 (1980).
Southern, E. M. J. molec. Biol. 98, 503–517 (1975).
Forsgren, B., Gustafsson, J.-A., Pousette, A. & Hogberg, B. Cancer Res. 39, 5155–5164 (1979).
Jönsson, G., Hogberg, B. & Nilsson, T. Scand. J. Urol. Nephrol. 11, 231–238 (1977).
Birnboim, H. C. & Doly, J. Nucleic Acids Res. 7, 1513–1523 (1979).
Paterson, B. M., Roberts, B. E. & Kuff, E. L. Proc. natn. Acad. Sci. U.S.A. 74, 4370–4374 (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Parker, M., Needham, M. & White, R. Prostatic steroid binding protein: gene duplication and steroid binding. Nature 298, 92–94 (1982). https://doi.org/10.1038/298092a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/298092a0
This article is cited by
-
Production of monoclonal antibody against the C1 component of rat estramustine-binding protein: Immunohistochemical study of rat prostate
Histochemistry and Cell Biology (1995)
-
The amino acid sequence of the alpha subunit of mouse salivary androgen-binding protein (ABP), with a comparison to the partial sequence of the beta subunit and to other ligand-binding proteins
Biochemical Genetics (1993)
-
Production of an antibody to mouse salivary androgen binding protein (ABP) and its use in identifying a prostate protein produced by a gene distinct from Abp
Biochemical Genetics (1986)
-
Ultrastructural localization of uteroglobin immunoreactivity in rabbit lung and endometrium, and rat ventral prostate
Histochemistry (1985)
-
Multiple gene action determining a mouse salivary protein phenotype: Identification of the structural gene for androgen binding protein (Abp)
Biochemical Genetics (1984)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.
