Abstract
Deletions were generated in vitro in the leader region of the tryptophan operon of Serratia marcescens and subsequently incorporated into the Escherichia coli chromosome in single copy form. Deletions which removed the translation start codon for the leader peptide or which ended in the ribosome recognition region preceding the leader peptide coding segment, caused super attenuation, that is, increased transcription termination at the attenuator. Apparently, the capacity to initiate translation of this coding region modulates expression of the operon.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Jackson, E. & Yanofsky, C. J. molec. Biol. 76, 89–101 (1973).
Lee, F. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 74, 4365–4369 (1977).
Lee, F., Bertrand, K., Bennett, G. & Yanofsky, C. J. molec. Biol. 121, 193–217 (1978).
Miozzari, G. F. & Yanofsky, C. Nature 276, 684–689 (1978).
Miozzari, G. F. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 75, 5580–5584 (1978).
Zurawski, G., Elseviers, D., Stauffer, G. V. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 75, 5988–5992 (1978).
Gray, H. B. et al. in Gene Analysis and Amplification Vol. 2 (eds Chinkjain, J. G. & Papas, T. S.) (North Holland, Amsterdam, 1981).
Maxam, A. M. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1974).
Shine, J. & Dalgarno, L. Proc. natn. Acad. Sci. U.S.A. 71, 1342–1346 (1974).
Stroynowski, I. & Yanofsky, C. Nature 298, 34–38 (1982).
Winkler, M. E. & Yanofsky, G. Biochemistry 20, 3738–3744 (1981).
Johnston, H. M. & Roth, J. R. J. molec. Biol. 145, 713–734 (1980).
Johnston, H. M. & Roth, J. R. J. molec. Biol. 145, 735–756 (1980).
Rose, J. K. and Yanofsky, C. J. molec. Biol. 69, 103–118 (1972).
Schmeissner, V., Ganem, D. & Miller, J. H. J. molec. Biol. 109, 303–326 (1977).
Miozzari, G. F. & Yanofsky, C. J. Bact. 133, 1457–1466 (1978).
Chang, A. C. Y. & Cohen, S. J. Bact. 134, 1141–1156 (1978).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stroynowski, I., van Cleemput, M. & Yanofsky, C. Superattenuation in the tryptophan operon of Serratia marcescens. Nature 298, 38–41 (1982). https://doi.org/10.1038/298038a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/298038a0
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.