Abstract
We have analysed the regulatory behaviour of deletion mutants lacking different segments of the leader region of the tryptophan operon of Serratia marcescens. Our results support the model in which a particular RNA structure, the terminator, is recognized during transcription as a transcription termination signal, and an alternative RNA structure, the anti-terminator, prevents formation of the terminator. It appears that the role of translation, ribosome stalling and shifts between alternative RNA secondary structures, is simply to regulate formation of the terminator.
This is a preview of subscription content, access via your institution
Access options
Subscribe to this journal
Receive 51 print issues and online access
$199.00 per year
only $3.90 per issue
Buy this article
- Purchase on Springer Link
- Instant access to full article PDF
Prices may be subject to local taxes which are calculated during checkout
Similar content being viewed by others
References
Crawford, I. P. & Stauffer, G. V. A. Rev. Biochem. 49, 163–195 (1980).
Yanofsky, C. Nature 289, 751–758 (1981).
Platt, T. Cell 24, 10–23 (1981).
Lee, F. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 74, 4365–4369 (1977).
Stauffer, G. V., Zurawski, G. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 75, 4833–4837 (1978).
Zurawski, G., Elseviers, D., Stauffer, G. V. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 75, 5988–5992 (1978).
Oxender, D., Zurawski, G. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 76, 5524–5528 (1979).
Zurawski, G., Brown, K., Killingly, D. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 75, 4271–4275 (1978).
Johnston, H. M., Barnes, W. M., Chumley, F. G., Bossi, L. & Roth, J. R. Proc. natn. Acad. Sci. U.S.A. 72, 508–512 (1975).
Gardner, J. F. Proc. natn. Acad. Sci. U.S.A. 76, 1706–1710 (1979).
Gemmill, R. M., Wessler, S. R., Keller, E. B. & Calvo, J. R. Proc. natn. Acad. Sci. U.S.A. 76, 4941–4945 (1979).
Lawther, R. P. & Hatfield, G. W. Proc. natn. Acad. Sci. U.S.A. 77, 1862–1866 (1980).
Nargang, F. E., Subrahmanyam, C. S. & Umbarger, H. E. Proc. natn. Acad. Sci. U.S.A. 77, 1823–1827 (1980).
Manson, M. & Yanofsky, C. J. Bact. 126, 679–689 (1976).
Hutchinson, M. A. & Belser, W. L. J. Bact. 98, 109–115 (1969).
Largen, M. & Belser, W. L. Genetics 75, 19–22 (1973).
Miozzari, G. F. & Yanofsky, C. Nature 276, 684–689 (1978).
Stroynowski, I., van Cleemput, M. & Yanofsky, C. Nature 298, 38–41 (1982).
Tinoco, I. et al. Nature new Biol. 246, 40–41 (1973).
Zurawski, G. & Yanofsky, C. J. molec. Biol. 142, 123–129 (1980).
Winkler, M. E., Mullis, K., Barnett, J., Stroynowski, I. T. & Yanofsky, C. Proc. natn. Acad. Sci. U.S.A. 79, 2181–2185 (1982).
Johnston, H. M. & Roth, J. R. J. molec. Biol. 145, 713–734 (1980).
Johnston, H. M. & Roth, J. R. J. molec. Biol. 145, 735–756 (1980).
Chang, A. C. Y. & Cohen, S. J. Bact. 134, 1141–1156 (1978).
Nogel, H. & Bonner, D. M. J. biol. Chem. 218, 97–106 (1956).
Zalkin, M. & Cling, D. Biochemistry 7, 3566–3573 (1968).
Maxam, A. & Gilbert, W. Proc. natn. Acad. Sci. U.S.A. 74, 560–564 (1977).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stroynowski, I., Yanofsky, C. Transcript secondary structures regulate transcription termination at the attenuator of S. marcescens tryptophan operon. Nature 298, 34–38 (1982). https://doi.org/10.1038/298034a0
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1038/298034a0
This article is cited by
-
ThepheA/tyrA/aroF region fromErwinia herbicola: An emerging comparative basis for analysis of gene organization and regulation in enteric bacteria
Journal of Molecular Evolution (1993)
-
Superattenuation in the tryptophan operon of Serratia marcescens
Nature (1982)
Comments
By submitting a comment you agree to abide by our Terms and Community Guidelines. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate.